TITULACIÓN: European Master of Sciences in Nematology (EUMAINE) CURSO ACADÉMICO: 2010-2011 GUÍA DOCENTE 1. DATOS BÁSICOS DE LA ASIGNATURA NOMBRE: Biological control of plant parasitic nematodes CÓDIGO1: TIPO: Créditos ECTS: 6 FECHAS IMPARTICIÓN2: CURSO ACADÉMICO: 2010-2011 November 22, 2010 – December 3, 2010 2. DATOS BÁSICOS DEL PROFESORADO NOMBRE: Soledad Verdejo Lucas CENTRO/DEPARTAMENTO: INSTITUCIÓN: Institut de Recerca i Tecnologia Agroalimèntaries (IRTA), Barcelona ÁREA: Nº DESPACHO: URL WEB: E-MAIL: soledad.verdejo@irta.es TLF: NOMBRE: Miguel Talavera Rubia CENTRO/DEPARTAMENTO: INSTITUCIÓN: IFAPA Centro Camino de Purchil (Granada) ÁREA: Nº DESPACHO: URL WEB: E-MAIL: miguelf.talavera@juntadeandalucia.es TLF: 3. PRERREQUISITOS, CONTEXTO Y RECOMENDACIONES PRERREQUISITOS: Students should have a basic academic knowledge of biology and a good knowledge of plantparasitic nematodes, which is provided during the 1st year’s major Nematology applied to agro-ecosystems. Students should have followed at least the courses on General Morphology and General Techniques in Nematology. The courses on Quantitative Plant Nematology, Temperate Nematology and Tropical Nematology are also recommendable. CONTEXTO DENTRO DE LA TITULACIÓN: The course will be taught every year during the third semester (first semester of the second year) of the master. Root-knot nematodes, Meloidogyne spp, are the main limiting factor for vegetable production worldwide. Conventionally, control of Meloidogyne has been based on the use of soil fumigant nematicides. However, current restrictions in the use of agro-chemicals have driven research efforts towards biological alternative methods. Biological control is an important tool for pest and disease management but additional field data is a need. Numerous organisms have been found associated to plant-parasitic nematodes including fungi, bacteria, and predacious nematodes. The three organisms most widely investigated for biological control of nematodes are Pasteuria penetrans, Pochonia chamidosporia, and Paecilomyces lilacinus. This course will provide knowledge on biological control of nematodes. Students will learn about their life cycles, mode-of action, and biological control potential. In addition, they will learn how to identify these organisms and assess their efficacy under experimental conditions. RECOMENDACIONES Y ADAPTACIONES CURRICULARES: 4. COMPETENCIAS Y RESULTADOS DE APRENDIZAJE Código 1.3 Denominación de la competencia Possess the attitude to use supporting sciences in professional activities and/or independent research and to use these constructively. 1.4 Investigate and understand interaction with other relevant science domains and integrate them within the context of more advanced ideas and practical applications and problem solving. 2.2 Recognize nematological problems in developing countries and be creative/inventive to tackle these problems. 2.6 Collect, register, process, analyse in a quantitative and structured way the collected data. 2.7 Assimilate, analyse, critically evaluate and synthesize information in a structured way from published international scientific literature and complex information sources. Demonstrate problem-prevention and problem-solving abilities, and use them in diverse situations or in a non-familiar context. 3.5 5.1 6.4 Resultado 1 Resultado 2 Resultado 3 Make connections/relationships between the personal scientific discipline and the society/public, focusing on questions and concerns, necessities and innovative needs that originate from within the society and relate these into an international context. Take an active and independent role in higher education, research, industry, extension service, quarantine agencies or administration and use the most recent information and communicate his/her expertise and knowledge. Resultados de aprendizaje Isolate Biological Control Organisms of nematodes Identify the main BCO of nematodes, Pasteuria, Paecilomyces and Pochonia Use laboratory techniques for isolation and culture of BCO of nematodes. 5. CONTENIDOS The course is divided in two domains: Theoretical and Practical Theoretical lectures on the following topics: Overview on the major groups of Biological Control Organisms (BCO), parasitic fungi, parasitic bacteria, nematode trapping fungi, nematode predators Bacterial parasites: The case of Pasteuria penetrans Fungal parasites: Pochonia chamidosporia and Paecilomyces lilacinus Biological Protection Organisms (BPO): Arbuscular Mychorrizal Fungae (AMF), Plant growth promoting bacteria (PGPR). Practical exercises Isolation and identification of nematode antagonist microorganisms Assessing potential of nematode antagonist as biological control agents under experimental conditions Visits to greenhouse and field experiments 6. METODOLOGÍA Y ACTIVIDADES ACTIVIDADES HORAS PRESENCIALES HORAS DE TRABAJO AUTÓNOMO TOTAL DE HORAS 15 45 60 35 85 120 50 130 180 Classroom lectures Guided lab exercises TOTALES: CRÉDITOS COMPETENCIAS ECTS (Códigos) 6 7. SISTEMA DE EVALUACIÓN A continuing evaluation method (see below) will be carried out throughout the duration of the course as well as a final exam at the end of the semester, certainly at the beginning of January. The lecturer will take note periodically of the work and progress of the student, taking into account his/her following of the activities and his/her improvement. Value: 50% of final score. The final exam will consist of the resolution of practical exercices: identification of problem specimens, description of one species, etc. Value: 50% of final score. 8. DOCUMENTACIÓN/BIBLIOGRAFÍA ESPECÍFICA o BÁSICA: CHEN, Z.X., CHEN, S.Y. & DICKSON, D.W. (2004). Nematology, Advances and Perspectives. Vol. 2. Nematode management and utilization. CABI Publishing.1234pp. CIANCIO, A. & MUKERJI, K.G. (2008). Integrated management and biocontrol of vegetable and grain crops nematodes. Springer. 356pp. KERRY, B.R. & CRUMP, D.H. (1991). Methods for studying nematophagous fungi. IOBC/WPRS Bulletin XIV/2 pp 64. LUC, M., SIKORA, R. & BRIDGE, J. (2005). Plant parasitic nematodes in subtropical and tropical agriculture. 2nd edition. CABI Publishing. 817pp. SOUTHEY, J.F. (1970). Laboratory methods for work with plant and soil nematodes. HMSO.Universidad de California. 202pp. STIRLING, G.R. (1991). Biological control of plant-parasitic nematodes. CAB International. Wallingford (Reino Unido). 282pp.