1. Course details - Universidad de Murcia

COURSE SYLLABUS MASTER DEGREE "REPRODUCTIVE
BIOTECHNOLOGIES AND RECOVERY OF ENDANGERED SPECIES" 2013/2014
1. Course details
1.1. The Course
Academic Year
2013/2014
MÁSTER UNIVERSITARIO EN
Degree
BIOLOGÍA Y TECNOLOGÍA DE LA
REPRODUCCIÓN EN MAMÍFEROS
REPRODUCTIVE BIOTECHNOLOGIES AND
Course Title
RECOVERY OF ENDANGERED SPECIES
Code
6098
Year
FIRST
Type
ELECTIVE COURSE
Number of groups
1
ECTS
3
Estimated student workload
75
Term taught
2º Cuatrimestre
SPANISH : Group 1
Language
ENGLISH : Group 1
Type of teaching
Campus-based course
1.2. The Teaching Staff
1
COURSE SYLLABUS MASTER DEGREE "REPRODUCTIVE
BIOTECHNOLOGIES AND RECOVERY OF ENDANGERED SPECIES" 2013/2014
Course
Area/Department
Coordinator
Staff position
FRANCISCO
E-mail Address /
FISIOLOGÍA VETERINARIA/ FISIOLOGÍA
PROFESOR CONTRATADO DOCTOR TIPO A (DEI)
fagarcia@um.es
ALBERTO GARCIA Website / Online
VAZQUEZ
Group: 1
http://www.um.es/grupo-fisiovet
office hours
Phone number,
Office hours and
Online office hours: YES
Term
Day
Office Hours
Location
Terms 1 and 2
Jueves
9:00- 11:00
868888009,
Location
Facultad de
Veterinaria
B1.1.040
MARIA PILAR
Area/Department
COY FUSTER
Staff position
Group: 1
FISIOLOGÍA VETERINARIA/ FISIOLOGÍA
CATEDRATICOS DE UNIVERSIDAD
E-mail Address /
pcoy@um.es
Website / Online
http://www.um.es/grupo-fisiovet
office hours
Phone
number,
Online office hours: YES
Term
Day
Office Hours
Location
Office hours and
Location
Additional
information
Terms 1 and 2
Lunes
11:00- 13:00
868884789,
Coordinadora
Facultad de
Veterinaria
B1.1.044
Terms 1 and 2
Miércoles
11:00- 13:00
868884789,
Facultad de
Veterinaria
B1.1.044
JOAQUIN
JERONIMO
Area/Department
Staff position
GADEA MATEOS E-mail Address /
Group: 1
Website / Online
office hours
FISIOLOGÍA VETERINARIA/ FISIOLOGÍA
PROFESORES TITULARES DE UNIVERSIDAD
jgadea@um.es
http://www.um.es/grupo-fisiovet
Online office hours: YES
2
COURSE SYLLABUS MASTER DEGREE "REPRODUCTIVE
BIOTECHNOLOGIES AND RECOVERY OF ENDANGERED SPECIES" 2013/2014
Phone
number,
Office hours and
Term
Day
Office Hours
Location
Terms 1 and 2
Jueves
10:00- 12:00
868884655,
Location
Facultad de
Veterinaria
2. Course Description
“Reproductive biotechnologies and recovery of endangered species” subject will allow to the students acquire
the competences about the main reproductive biotechnology methods such as artificial insemination, embryo
transfer or oestrus control between others, mainly conducted to veterinarians.
3. Course access requirements
3.1 Incompatibilities
3.2 Recommended background
Previous knowledge about the techniques most commonly used in asistant reproduction
4. Course-specific skills and their relation to degree-specific skills
Skill 1. To know the main reproductive biotechnologies applied to farm animals.
· Conseguir un conocimiento profundo y actualizado de las bases anatómicas, fisiológicas e histológicas del proceso reproductor en
los mamíferos en general, y más concretamente en la especie humana y en los animales de interés zootécnico (especies bovina,
porcina, equina, caprina y ovina).
Skill 2. Status of animal endangered species.
· Conseguir un conocimiento profundo y actualizado de las bases anatómicas, fisiológicas e histológicas del proceso reproductor en
los mamíferos en general, y más concretamente en la especie humana y en los animales de interés zootécnico (especies bovina,
porcina, equina, caprina y ovina).
Skill 3. To know the main reproductive biotechnologies applied to the recovery and conservation of endangered species
· Conseguir un conocimiento profundo y actualizado de las bases anatómicas, fisiológicas e histológicas del proceso reproductor en
los mamíferos en general, y más concretamente en la especie humana y en los animales de interés zootécnico (especies bovina,
porcina, equina, caprina y ovina).
Skill 4. To acquire skills in oestrus control, artificial insemination and embryo transfer techniques
· Ser capaz de desenvolverse en un laboratorio de reproducción humana o animal y podrá realizar por sí mismo técnicas como
la preparación de medios de cultivo, manipulación de gametos, inseminación artificial, maduración in vitro, fecundación vitro,
inyección intracitoplasmática de espermatozoides, cultivo de embriones, transferencia de embriones, crioconservación de gametos
y embriones, diagnósticos de ovulación o gestación por ultrasonografía, análisis espermáticos, manejo de bases de datos, estudios
citogenéticos, etc.
Skill 5. Being able to synthesize, prepare and discuss a research or professional theme about reproductive
biotechnologies
This course-specific skill is not related to any degree-specific skill.
5. Course contents
Part 1: Presentation
3
COURSE SYLLABUS MASTER DEGREE "REPRODUCTIVE
BIOTECHNOLOGIES AND RECOVERY OF ENDANGERED SPECIES" 2013/2014
UNIT 1 Presentation of the subject and the teaching guide
Part 2: Applied reproductive biotechnology
UNIT 1 Current state of the reproductive biotechnologies
UNIT 2 Sex sorted semen. Generalities
UNIT 3 Oestrous synchronization
UNIT 4 Embryo transfer
UNIT 5 Artificial insemination
Part 3: Recovery of endangered species: mammals
UNIT 1 Recovery of native breeds
UNIT 2 Introduction to reproductive technologies applied to wildlife
UNIT 3 Reproductive technologies in mountain ruminants
Part 4: Reprodctive biotechnologies applied in non mammal animals
UNIT 1 Generalities of reproductive biology in birds and reptiles
UNIT 2 Assistant reproduction in birds and reptiles
PRACTICES
Practice 1 Artifcial insemination :Related Content Unit 1 (Bloque 2),Unit 3 (Bloque 2) and Unit 5 (Bloque 2)
Artificial inseminations will be performed in some species (pigs, horses, etc) using different techniques. In
adittion, it will be included a visit to an artificial insemination center. Depending on the number of students
enrolled, the practice will take place in one group or divided in different student groups.
Practice 2 Management and handling of wild animals. Reproductive methodologies used in
zoos. :Related Content Part 3 and Part 4
This practice is based on a visit to a wildlife park, where the handling of animals will be explained and
reproductive guidelines applied as well. The visit will take place in one group.
6. Teaching methods and contact hours
Attendance Independent
Learning Activity
Methodology
Workload
Hours
Learning
13
26
The theoretical sessions will be held in the classroom
-1.2, Faculty of Veterinary Medicine. In these
Theorical sesions
sessions the lecture with cooperative learning
39
techniques will be combined with discussions of
results of research articles and projected images.
4
COURSE SYLLABUS MASTER DEGREE "REPRODUCTIVE
BIOTECHNOLOGIES AND RECOVERY OF ENDANGERED SPECIES" 2013/2014
Attendance Independent
Learning Activity
Methodology
Workload
Hours
Learning
8
12
20
16
16
Practices will take place in different livestock
Practical sessions
farms depending on their availability.
Students will perform a work based on the selection
Work and exposition
of a species/breed in endangered. The work will be
performed either individually or in groups of 2 students.
7. Course Timetable
http://www.um.es/web/veterinaria/contenido/estudios/masteres/bio-tecno-mamiferos/2013-14#horarios
8. Assessment
Methods / Instruments Exam with theorical contents
Evaluated skill Evaluation Criteria
1, 2, 3, 4
Examination with short and/or multiple choice questions.The exam is scored
from 0 to 10.
Weight
45
Methods / Instruments Written work and presentation
Evaluation Criteria
The student will carry out a written work based on the current situation of a
Evaluated skill
endangered species/breed. In addition, the students will have 10 minutes for
5
a presentation of the mentioned work. The final score will be the sum of the
written document score (0-5 points) and the presentation score (0-5 points).
Weight
45
Methods / Instruments Attendance and participation
Evaluated skill Evaluation Criteria
1, 2, 3, 4, 5
Assistance and participation during the theorcial and practical sessions will be
highly valued.
Weight
10
Exam date
http://www.um.es/web/veterinaria/contenido/estudios/masteres/bio-tecnomamiferos/2013-14#examenes
5
COURSE SYLLABUS MASTER DEGREE "REPRODUCTIVE
BIOTECHNOLOGIES AND RECOVERY OF ENDANGERED SPECIES" 2013/2014
9. Reading list (required and recommended readings)
YANAGIMACHI, R. (2001): «Gamete manipulation for development: New methods for conception»,
Reproduction Fertility and Development, 13, 3-14.
BARNES, F. L. (2003): «In vitro oogenesis», en Assisted Reproductive Technology. Accomplishments
and New Horizons. C.J. de Jonge y C. L. R. Barratt (Eds.), Cambridge, Cambridge University Press,
págs. 61-69.
BUCKRELL, B., et al. (1990): Failure to maintain interspecific pregnancy after tranfer of Dall"s sheep
embryos to domestic ewes. Journal of Reproduction and Fertility 90, 387-394.
CABODEVILA, J. y M. TERUEL (2001): Criopreservación de embriones bovinos. En: Biotecnología de la
Reproducción. G. A. Palma (Ed.), Balcarce, Ediciones INTA, págs. 149-174.
CASSINELLO, et al. (1998): «Characteristics of the semen of three endangered species of gazelles
(Gazella dama mhorr, G. dorcas neglecta and G.cuvieri)», Journal of Reproduction and Fertility, 113,
35-45.
CHEN, D. Y. et al. (1999): «The giant panda (Ailuropoda melanoleuca) somatic nucleus can
dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg», Science in
China (series C), 42, 346-353.
CIBELLI, J. et al. (2002): Principles of Cloning, Amsterdam, Academic Press.
CRITSER, J. K., L. K. RILEY y R. S. PRATHER (2003): «Application of nuclear transfer technology
to wildlife species», en Reproductive Science and Integrated Conservation. W. V. Holt et al. (Eds.),
Cambridge, Cambridge University Press, págs. 195-208.
CRITSER, J. K., Y. AGCA y E. J. WOODS (2003): Cryopreservation of immature and mature gametes.
En: Assisted Reproductive Technology. Accomplishments and New Horizons. C. J. de Jonge y C. L. R.
Barratt (Eds.), Cambridge, Cambridge University Press, págs. 144-166.
GARDE, J. et al. (1995): «Live birth of hybrid (O. Musimon x O. aries) lambs following intrauterine
insemination in domestic sheep with mouflon semen obtained 40 hours postmortem», Theriogenology,
43, 218-225.
HOLT, W. V. (2001): «Germplasm cryopreservation in elephants and wild ungulates», en Cryobanking
the Genetic Resource, P. F. Watson y W. V. Holt (Eds.), London, Taylor & Francis, págs. 317-348.
IRITANI, A., Y. HOSOI y R. TORII (1998): «Application of ICSI in domestic and/or zoo animals», en
Gametes: Development and Function, A. Lauria et al. (Eds.), Roma, Serono Symposia, págs. 393-404.
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COURSE SYLLABUS MASTER DEGREE "REPRODUCTIVE
BIOTECHNOLOGIES AND RECOVERY OF ENDANGERED SPECIES" 2013/2014
JOHNSON, L. A. y G. R. WELCH (1999): «Sex preselection: high-speed flow cytometric sorting of X and
Y sperm for maximum efficiency», Theriogenology, 52,
LANZA, R. P. et al. (2000b): «Cloning of an endangered species (Bos gaurus) using interspecies nuclear
transfer», Cloning, 2, 79-90.
LOI, P. et al. (2001): «Genetic rescue of an endangered mammal by cross-species nuclear transfer using
post-mortem somatic cells», Nature Biotechnology, 19, 962-964.
LOSKUTOFF, N. M. (1998): «Biology, technology and strategy of genetic resource banking in
conservation programs for wildlife», en Gametes: Development and Function. A. Lauria et al. (Eds.),
Roma, Serono Symposia, págs. 275-286.
LOSKUTOFF, N. y K. BETTERIDGE (1993): Embryo technology in pets and endangered species. En:
Embryonic development and manipulation in animal production: trends in research and applications. A.
Lauria y F. Gandolfi (Eds.), London, Portland Press, págs. 235-248.
MONFORT, S. L. et al. (1993): «Successful intrauterine insemination of Eld"s deer (Cervus eldi thamin)
with frozen-thawed spermatozoa», Journal of Reproduction and Fertility, 99, 459-465.
POPE, C. E. (2000): «Embryo technology in conservation efforts for endangered felids», Theriogenology,
53, 163-174.
PTAK, G. et al. (2002): «Preservation of the wild European mouflon: the first example of genetic
management using a complete program of reproductive biotechnologies », Biology of Reproduction, 66,
796-801.
Rao M, Saw Htun, Platt SG, Tizard R, Poole C, Than Myint, Watson JE. Biodiversity conservation in a
changing climate: a review of threats and implications for conservation planning in Myanmar.Ambio. 2013
Nov;42(7):789-804. doi: 10.1007/s13280-013-0423-5. Epub 2013 Jul 19.
ROLDAN, E. R. S. et al. (1998): «Inbreeding, fluctuating asymmetry and ejaculate quality in an
endangered ungulate», Proceedings of the Royal Society of London, series B, 265, 243-248.
SEIDEL, G. E. (2003): «Economics of selecting for sex: the most important genetic trait»,
Theriogenology, 59, 585-598.
SOLER, A. J. y J. GARDE (2003): «Relationship between the characteristics of epididymal red deer
spermatozoa and penetrability into zona-free hamster ova», Journal of Andrology, 24, 393-400.
SUMMERS, P. et al. (1987): «Successful transfer of the embryo of Przewalski"s horse (Equus
przewalskii) and grant"s zebra (E. burchelli) to domestic mares (E. caballus)», Journal of Reproduction
and Fertility, 80, 13-20.
WATSON, P. F. y W. V. HOLT (Eds.) (2001): Cryobanking the Genetic Resource, London, Taylor and
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COURSE SYLLABUS MASTER DEGREE "REPRODUCTIVE
BIOTECHNOLOGIES AND RECOVERY OF ENDANGERED SPECIES" 2013/2014
Francis.
WHITE, K. L. et al. (1999): «Establishment of pregnancy after the transfer of nuclear transfer embryos
produced from the fusion of argali (Ovis ammon) nuclei into domestic sheep (Ovis aries) enucleated
oocytes», Cloning, 1, 47-54.
WILDT, D. E. (1990): «Potential applications of IVF technology for species conservation », en
Fertilization in Mammals, B. D. Bavister, J. M. Cummins y E. R. S. Roldan (Eds.), Norwell, MA, Serono
Symposia, págs. 349-364.
AVARBOCK, M. R., C. J. BRINSTER Y R. L. BRINSTER (1996): Reconstituition of spermatogenesis
from frozen spermatogonial stem cells. Nature Medicine 2, 693-696.
Lifewild conservation
Wild spirit
10. Additional information
8