IBA Headquarters IBA GmbH Rudolf-Wissell-Str. 28 37079 Goettingen Germany Tel. +49 (0) 551-5 06 72-0 Fax +49 (0) 551-5 06 72-181 Data Sheet IBA US Distribution Center 1328 Ashby Road Olivette, MO 63132 USA Tel. 1-877-IBA-GmbH (1-877-422-4624) Fax 1-888-531-6813 Ni-NTA Superflow® E-mail: info@iba-lifesciences.com http://www.iba-lifesciences.com Cat. No.: Lot No.: 2-3206-010, 2-3206-025, 2-3206-100, 2-3206-500 3206- Last date of revision March 2012 Version 3206-7 Description For purification of 6xHistidine-tag proteins by gravity flow and FPLC. Ni-NTA Superflow combines superior mechanical stability and outstanding flow characteristics with high dynamic capacity. This resin allows purification of 6xHistidinetag proteins under gravity flow as well as high flow rates and pressures for efficient FPLC applications. Support Superflow, highly cross-linked, 6% agarose Bead size 60–160 µm Form 50 % suspension in 30 % ethanol, pre-charged with Ni Binding capacity Up to 50 mg/ml (up to 2500 nmol @ ~20 kDa) Storage 4 °C Shipment Room temperature 2+ Ni-NTA Sepharose: Flammable. Harmful if swallowed. Possible risks of irreversible effects. May cause sensitization by inhalation and skin contact. Keep away from food, drink and animal feedingstuffs. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing. If swallowed, seek medical advice immediately and show this container or label. Ni-NTA Sepharose: Entzündlich. Gesundheitsschädlich beim Verschlucken. Irreversibler Schaden möglich. Sensibilisierung durch Einatmen und Hautkontakt möglich. Von Nahrungsmitteln, Getränken und Futtermitteln fernhalten. Berührung mit den Augen vermeiden. Bei der Arbeit geeignete Schutzkleidung tragen. Bei Verschlucken sofort ärztlichen Rat einholen und Verpackung oder Etikett vorzeigen. Ni-NTA Sepharose: R10-22-40-42/43, S13-26-36-46 Harmful, Sensitizer, Flammable Contains nickel-nitrilotriacetic acid, ethanol Ni-NTA Sepharose: Inflammable. Nocif en cas d'ingestion. Possibilité d'effets irréversibles. Peut entraîner une sensibilisation par inhalation et par contact avec la peau. Conserver à l'écart des aliments et boissons, y compris ceux pour animaux. En cas de contact avec les yeux, laver immédiatement et abondamment avec de l'eau et consulter un spécialiste. Porter un vêtement de protection approprié. En cas d'ingestion, consulter immédiatement un médecin et lui montrer l'emballage ou l'étiquette. Ni-NTA Sepharose: Infiammabile. Nocivo per ingestione. Possibilità di effetti irreversibili. Può provocare sensibilizzazione per inalazione e contatto con la pelle. Conservare lontano da alimenti o mangimi e da bevande. In caso di contatto con gli occhi, lavare immediatamente e abbondantemente con acqua e consultare un medico. Usare indumenti protettivi adatti. In caso d'ingestione consultare immediatamente il medico e mostrargli il contenitore o l'etichetta. Ni-NTA Sepharose: Inflamable. Nocivo por ingestión. Posibilidad de efectos irreversibles. Posibilidad de sensibilización por inhalación y en contacto con la piel. Manténgase lejos de alimentos, bebidas y piensos. En caso de contacto con los ojos, lávense inmediata y abundantemente con agua y acúdase a un médico. Úsese indumentaria protectora adecuada. En caso de ingestión, acúdase inmediatamente al médico y muéstresele la etiqueta o el envase. For research use only For research use only The Ni-NTA resin contained in this Product is manufactured by QIAGEN under a license from Hoffmann-LaRoche Inc., Nutley, NJ and/or Hoffmann-LaRoche Ltd., Basel, Switzerland and is provided only for use in research. Information about licenses for commercial use is available from QIAGEN GmbH, Max-Volmer-Strasse 4, D-40724 Hilden, Germany.