Pruebas de confirmación de microorganismos

Pruebas de confirmación
de microorganismos
Bioser, S.A. le ofrece sistemas simples y de confianza para laboratorios clínicos, alimentarios y farmacéuticos.
Galerías de identificación Bioquímica
Características:
• Disponibles todos los reactivos necesarios
• Sustratos basados en las pruebas convencionales:
correlación directa con métodos estándares.
• Software de Identificación con muchas funciones
y fácil manejo
Código
Descripción
Formato
Beneficios:
025022020
Microgen Listeria ID
20 test
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025020060
Microgen GN A ID
60 test
025021024
Microgen GN B ID
24 test
025033020
Microgen Bacillus ID
20 test
025034020
Microgen Staph ID
20 test
025041020
Microgen Strep ID
20 test
Sistema flexible
Ahorro en el coste
Ahorro en el espacio
Ahorro en el tiempo
Fácil de usar
Test de aglutinación de Latex
Características:
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Resultados en dos minutos
Eficiencia incrementada
Elevada sensibilidad
Elevada especificidad
Resultados exactos y fiables
Un solo paso
Código
Descripción
Formato
025009050
Microgen Legionella latex
50 test
025010050
Microgen E.coli O:157 latex
50 test
025012050
Microgen Campylobacter latex
50 test
025014050
Microgen Salmonella latex
50 test
025016100
Microgen Staph latex
100 test
025018050
Microgen Strep latex
50 test
025015050
Microgen Listeria latex
50 test
C/ Tarragona, 106 · 08015 Barcelona · SPAIN · tel +34 93 226 44 77 · fax +34 93 226 79 79 ; e-mail: bioser@bioser.com · www.bioser.com
Guidelines for the Interpretation of the New Haemolysis Test in the Microgen Listeria-ID
Background
The AOAC-RI validated Microgen Listeria-ID has been improved to make the system the easiest-to-read, user-friendly and
cost-effective method of identifying Listeria species on the market today.
The Microgen Listeria-ID is a biochemical identification system which consists of 10 sugar fermentation tests, Aesculin
hydrolysis and an improved micro-haemolysis reaction. The system does not require any reagent additions and reduces
laboratory costs whilst utilising substrates which conform to all international standards.
Microgen Listeria-ID (product code: MID-67) has now been improved to provide the easiest to read method of differentiating
haemolytic and non-haemolytic Listeria spp..
Haemolytic and non-haemolytic Listeria spp. have traditionally been differentiated by the sub-culture from selective medium
to sheep blood agar for the detection of haemolytic activity or by performing the variable, complex and difficult to interpret
CAMP test. The inclusion of a sensitive and stable micro-haemolysis in the Microgen Listeria-ID allows for the easy
interpretation of haemolysis of the red blood cells by haemolytic Listeria spp..
Interpretation of the New Haemolysis Reaction
The haemolysis reaction in the MID Listeria is based on the determination of the ability of a test organism to lyse the red
blood cells contained in the haemolysis reagent contained with this test.
A STRONG POSITIVE HAEMOLYSIS TEST is typically characterized by the complete lysis of all of the red blood cells
contained within the haemolysis reagent. This is seen as the development of a cloudy straw brown suspension in the test
well and the absence of a deposit or button of cells in the bottom of the test well.
Typical POSITIVE REACTION (Total haemolysis)
A WEAK POSITIVE HAEMOLYSIS TEST is typically characterized by the incomplete lysis of the
red blood cells within the haemolysis reagent. This is seen as the development of a cloudy straw to
dark brown suspension in the test well and the presence of a small deposit or button of cells in the
bottom of the test well.
Typical WEAK POSITIVE REACTION (Partial haemolysis)
Weak positive haemolysis tests may occur with strains of L. monocytogenes and L. seeligeri.
Although L. monocytogenes generally produces a strong definitive haemolysis reaction, it is possible
for a strain to show a weak reaction. This is normally when the organism has been stressed e.g. from
environmental samples or if the strain has been resuscitated from storage. Weak haemolysis is
typically seen in weak producers such as L. seeligeri.
A NEGATIVE HAEMOLYSIS TEST is typically characterized by the absence of lysis of any of the red
blood cells contained within the haemolysis reagent. This is seen as the development of a clear straw
to very pale pink colour in the test well and the presence of a large opaque red to brown coloured
deposit or button of cells in the bottom of the test well.
Typical NEGATIVE REACTION (no haemolysis)
Procedural note:
Test strains that have been removed from storage (freeze dried or lenticules) should be considered to be ‘damaged’.
Optimum activity needs to be restored by inoculation into a suitable broth (e.g. TSYB) and incubated for 18-24 hours at
37°C. if a haemolytic strain, the presence of haemolysin should be confirmed by inoculating a blood plate and incubating for
18-24 hours at 37°C.
Bioser, S.A. c/Tarragona 106 · 08015 Barcelona · Tel: 93 226 44 77 · Fax: 93 226 79 79 · bioser@bioser.com · www.bioser.com