Conflict-of-interest statement To the best of our knowledge, no conflict of interest exists. The authors declare: No conflict of interest. The current experimental study did not receive any external funding or industry. There is no conflict of interest in performing this study by either of the authors. The material has been donated by the Department of General and Digestive Surgery of the Riotinto Hospital and Virgen del Rocío Hospital. University Hospital of Virgen del Rocío. Seville. Spain. Riotinto Hospital. Huelva. Spain