∆ ν ! " # $ ( , % ) ! & *& ) !' + $ * ) * - . / ) )0 * 4+ 1 * "2 3 -2 5 " $ 1 & & - Absorbancia H2O Ox Gly NH3 en 0 300 6 8 600 ! 9 900 nm 7 3 ! : 8 $ <= 98 3 1 ; 8 98 & 1 >>>> >>>> 6" &" ?" ∆Ε >>↑ ↑>> >>>> >>>> 6& 6? &? ν & @ 0 7 4 ν 0 + A < $ ! B CD %+ '"%; "8 '"E"2 CD %+ '-E"2 4 4 & F 0 ? @ $ & G 1 ∆4 ν ν 1 0 $ / / λ = H7 @ @$ 7 I8 58 J 3 /λ 0 %λ' ?0 & 7ν ' 0 7∆4 %0 & 7 %ε' ε 1 ε 0 0 K < 7 F + < Ψ Ψ+ ? α Ψ µ Ψ+ 4 6 + 0 ? 7 ε7 L < Ψ µ Ψ+ Ψ µ Ψ+ < $ ! )= ! + < $ 7 * $ ! )= 7 * % 7$ $ ' M ! < + 4549 <8 9 %; "8 'B"2 . %; "8 'B-2 ↑ ↑ A A ↑ ↑ ↑ Iε N M M- J ! ! + 7 ! < ! %$ $' >>>> 1 %; "8 'B"27% ? ' ε N "M 9 >>>> 6" &" ?" ) $ * >>↑ ↑>> >>>> >>>> 6& < 6? ) 7 &? * ) "$* D ? ε ≈ G:7MMM 7 9 " 1 1 :" ε A:M 1 %; "8 'B"2 ε A # D % φ-'"1 1 % '"1 "2 ε GK 1 % '"1 "2 ε -: " - ! 3 ! 7%$ 7 $' 4@ >> <= 98 %$' ε > M7MMM ↑↓ ; 8 98 % ' : !" # D $ 0 9O B % % $ &' 89 π& ' A 1 π7 % $ 78; ' _____ _____ _____3t1u _____2a1g p___ ___ ___ 1 O ) π* & _____ _____2eg () * + , )-. s___ d___ ___ ___ ___ ___ _____ _____ _____2t2g )- 8 9 π7 )- 8 / A @ $ _____ _____ _____t1g _____ _____ _____t2u _____ _____ _____2t1u ____________π 1 _____ _____ _____1t2g O __________σ _____ _____ 1eg _____ _____ _____1t1u _____ 1a1g σ & ?7 6" &"7 ?" "8/ 8 9σ 7 67 &7 %B2B "' " B π % O 78 ; '_____ 1 _____ _____3t1u _____2a1g $ p___ ___ ___ ____ ____2eg s___ d ↑↓ _↑↓ _↑↓ ___ ___ $$ _↑↓_ _↑↓_ _↑↓ 2t2g _↑↓__ _↑↓_ _↑↓_t1g _↑↓__ _↑↓_ _↑↓_t2u _↑↓__ _↑↓_ _↑↓_2t1u _↑↓__ _↑↓_ _↑↓_1t2g _ _ ↑↓ ↑↓ ↑↓ ↑↓ ↑↓ ↑↓ ↑↓ ↑↓ ↑↓ ↑↓ ↑↓ ↑↓ π ↑↓ ↑↓ ↑↓ ↑↓ ↑↓ ↑↓ σ _↑↓__ _↑↓_ 1eg _↑↓_ _↓↑_ _↑↓_ 1t1u __↑↓_ 1a1g G 1 π7 % 1 8 71 D ' //// //// //// //// ) ) 0 0 0 //// //// //// //// //// //// //// //// //// //// //// //// $ ) /// /// /// /// /// ///π0 /// /// /// /// /// ///π0 ) //// //// 0 /// /// /// /// ↑↓ ↑↓ ↑↓ ↑↓ ↑↓ ↑↓ σ /↑↓ ↑↓ /↑↓ /// /// $$ /↑↓ ↑↓ /↑↓ /↑↓/ /↑↓/ /↑↓/ /↑↓/ /↑↓ /↑↓/ ) ) K ) * 44 + < $ 0 ? L 4 ) CE 6 ∆Ε "$ $ CE ν "$ ν ∆Ε0 ν 2 0 % 0 ν $ =H '7 & # "M >>>>>>>>> >>>>>>>>> >>>>>>>>> >>>>>>>>> >>>>>>>>> >>>>>>>>> ) 6 ∆Ε 4 ν %= H 0 ' ∆Ε ν % #' + 0 6 ν ≠ ν" ≠ ν- ≠ ν: >>>>>>>>> >>>>>>>>> >>>>>>>>> >>>>>>>>> >>>>>>>>> >>>>>>>>> 0 + 0 " 1 2 3 2 45 "" 4 6 3 6 Ψ µ Ψ+ ) * M )= Ψ !7 Ψ * % Ψ 7$ $' Ψ Ψ 3 Ψ 0 Ψ ! 0 )0 $ * "- 1 + ) 4+ "2 3 -2 * 5 ": +* 6 7 * ↑↓ ↑ ↑↓ ↑↓ ↑ ↑↓ 3 %; "8 'B-2 + 1 %; "8 'B"2 + 7 ! "A 3 -2 1 "2 ↑ ↓↑ ↓↑ ↓↑ ↓↑ ↑ ν ν" %ν-' ν ν" %ν-' "B "G