REVISTA LATINOAMERICANA DE QUÍMICA EDITOR Dr. Andrés Navarrete The Revista Latinoamericana de Química is an international Journal. All manuscripts submitted are subject to peer review. Expert scientists in the subject areas being treated will evaluate all manuscripts for validity of all experimental procedure, originality, VLJQLÀFDQFHDQGDSSURSULDWHQHVVWRWKHMRXUQDO La Revista Latinoamericana de Química es una publicación internacional. Todos los manuscritos VRPHWLGRVVRQVXMHWRVDUHYLVLyQ&LHQWtÀFRVH[SHUWRVHQODViUHDVWUDWDGDVHYDOXDUiQWRGRV los manuscritos en cuanto a la validez de los procedimientos experimentales, originalidad, importancia y adecuación para la Revista. IXIM M Revista Editada por: Laboratorios Mixim, S.A. de C.V. Calle Jardín Sur No. 6, Apartado Postal 3, 1DXFDOSDQGH-XiUH](VWDGRGH0p[LFR0p[LFR /DUHYLVWDDFWXDOPHQWHHVWiLQFOXtGDHQODVVLJXLHQWHVEDVHVGHGDWRV&KHPLFDO$EVWUDFW0HGOLQH %LRVLV(%6&229,'3(5,2',&$&DPEULGJH6FLHQWLÀF,QIRUPDWLRQ1$35$/(578OULFK·V ,QWHUQDWLRQDO3HULRGLFDOV'LUHFWRU\$OH[DQGHU0DUNHWLQJ6HUYLFHV,QF8QLYHUVLW\RI&DOLIRUQLD Science Library Services, entre otras. MÉXICO 2013 29 a 31 de Mayo de 2013 Universidad Autónoma del Estado de Hidalgo Pachuca de Soto, Hidalgo México I Maestro Humberto Augusto Veras Godoy RECTOR DE LA UNIVERSIDAD AUTÓNOMA DEL ESTADO DE HIDALGO Dr. Orlando Ávila Posos DIRECTOR DEL INSTITUTO DE CIENCIAS BÁSICAS E INGENIERÍA, UAEH Dr. Carlos Andrés Galán Vidal JEFE DEL ÁREA ACADÉMICA DE QUÍMICA, ICBI, UAEH Dra. Noemí H. Waksman Minsky PRESIDENTA DE LA ASOCIACIÓN MEXICANA DE INVESTIGACIÓN EN PRODUCTOS NATURALES, A.C. COMITÉ ORGANIZADOR COMITÉ CIENTÍFICO Dr. J. Martín Torres Valencia Dr. J. Roberto Villagómez Ibarra Dr. Alejandro Álvarez Hernández Dr. Oscar R. Suárez Castillo Dra. Myriam Meléndez Rodríguez Dra. Maricruz Sánchez Zavala Dra. Susana Rojas Lima Dr. Heraclio López Ruiz Dra. Margarita Tlahuextl Romero Dr. Antonio R. Tapia Benavides Dr. Pedro Joseph-Nathan Dr. Carlos M. Cerda García Rojas Dra. Maricruz Sánchez Zavala M.C. Dora luz Quintero Mogica M.C. Araceli Sierra Zenteno Dra. Minarda de la O Arciniega Dr. Juan A. Gayosso De Lucio Dra. Mirandeli Bautista Ávila Dra. Georgina Almaguer Vargas Dra. Raquel Cariño Cortés Dra. Claudia Velázquez González Dr. Eleuterio Burgueño Tapia Dr. Juan D. Hernández Hernández Dra. Beatriz Hernández Carlos Dra. Guadalupe Hernández Linares Dra. María A. Fernández Herrera Dra. Noemí H. Waksman Minsky Dra. Verónica M. Rivas Galindo Dra. Maria Luisa Garduño Ramírez Dra. María de la Luz Miranda Beltrán Dra. Ana María Puebla Pérez Dr. Ramón E. Robles Zepeda Dr. Sergio E. Peraza Sánchez COMITÉ DE LOGÍSTICA Q. L. Adriana Ortiz León M.C. Alejandro Valdez Calderón M.C. René Velázquez Jiménez Q. 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+DEVDK0RKDPG=DOLODZDWL0DW5DVKLG.KR]LUDK6KDDUL-DOLIDK/DWLS0G1RUGLQ+M/DMLV\$EG0DQDI$OL 3HUWDQLND-7URS$JULF6FL &RUUDO$JXD\R 5 <DKLD ( &DUULOOR/ySH] $ \ *RQ]iOH]$JXLODU * -RXUQDO RI $JULFXOWXUDO DQG )RRG &KHPLVWU\± *DUFtD$UUHGRQGR$5RMDV0ROLQD$,EDUUD$OYDUDGR&\,JOHVLDV3ULHWR5-RXUQDORI([SHULPHQWDO ± 9DUJDV6iH]-$7pVLVSDUDREWHQHUHOWtWXORGH0pGLFR9HWHULQDULRʊ$ FWLYLGDGGHDOFDORLGHVWLSRWURSDQR SUHVHQWHVHQ6FKL]DQWKXVJUDKDPLLVREUHODFRQWUDFFLyQGHDRUWDGHUDWD9DOGLYLD&KLOH 1DYDUUHWH&DVWUR $ %DOGHUDV/ySH] -/ 0HGLQD&UX] / 'pFLJD&DPSRV 0 \ $OIDUR5RPHUR $ *XLyQGH3UiFWLFDV)DUPDFRORJtD,,0p[LFR') 147 &(678',2&203$5$7,92'(/$%,2$&&(6,%,/,'$',19,752< &$3$&,'$'$17,2;,'$17('((;75$&726<(1&$368/$'2'(0DQJLIHUD LQGLFD 6DQGRYDO*DOOHJRV(05DPtUH]0RUHQR(*D\RVVR'H/XFLR-)HUQiQGH]0DUWtQH] ($ODQtV*DUFtD()LODUGR.HUVWXSS66XPD\D0DUWtQH]07&DO]DGD0HQGR]D&& &DULxR&RUWpV5 3URJUDPDGH0DHVWUtDHQ&LHQFLDV%LRPpGLFDV\GHOD6DOXG ,QVWLWXWRGH&LHQFLDVGHOD6DOXG&HQWURGH ,QYHVWLJDFLRQHV4XtPLFDV8QLYHUVLGDG$XWyQRPDGHO(VWDGRGH+LGDOJR 6HFUHWDUtDGH,QYHVWLJDFLyQ\(VWXGLRVGH 3RVJUDGR8QLYHUVLGDG$XWyQRPDGH1D\DULW/DERUDWRULR0XOWLGLVFLSOLQDULR(VFXHOD6XSHULRUGH0HGLFLQD ,QVWLWXWR3ROLWpFQLFR1DFLRQDO0p[LFR')(PDLOLOH#KRWPDLOFRP El mango (Mangifera indica (MI)) es un cultivo de clima tropical y subtropical, considerado como una de las especies de mayor importancia económica, la pulpa del fruto se utiliza para la elaboración de jugos, néctares, conservas, etc., mientras que los residuos (cáscara, semilla y pulpa) no son aprovechados, debido a la ausencia de evidencias experimentales respecto a su valor nutricional. El objetivo de este trabajo fue determinar la bioaccesibilidad intestinal de compuestos antioxidantes y la capacidad antioxidante presente en tres productos de MI: el extracto etanólico de la hoja (EH), el residuo industrial (cáscara, semilla y pulpa) obtenido del despulpado (RD) y el RD secado por aspersión (RDA). La bioaccesibilidad intestinal se evaluó por un proceso enzimático digestivo in vitro seguido por un proceso de diálisis. La actividad antirradical fue cuantificada mediante los ensayos con DPPH• y ABTS.+. Los resultados indicaron una concentración de polifenoles extraibles de 196.67, 136.41 y 584.79 mg EAG/g en las muestras de RD, RDA y EH en la muestra original, respectivamente. Estos valores son similares a lo presentado en otros estudios en frutos (Ribeiro et al, 2008; Kunradi VFG et al. 2009). Después del proceso digestivo el contenido de polifenoles totales fue menor (141.014, 68.188 y 342.391mgEAG/g de muestra en RD, RDA y EH), sin embargo representan entre 50 y 70 % de los compuestos presentes en la muestra original, siendo similar a lo encontrado en otros trabajos (Ramírez-Moreno, 2011). Estos compuestos liberados y bioaccesibles a nivel intestinal se debe a que son liberados por la acción enzimática gastrointestinal, ya que se hidrolizan los enlaces no covalentes entre los grupos hidroxilo de los polifenoles y los grupos polares de las moléculas polisacaridas (González AGA. et al.; Hugo PC, 2011). Estos compuestos liberados por el proceso digestivo mantuvieron en gran medida la actividad antirradical medida por DPPH, en el RD y RDA (86.70 y 87.78 %), mientras que para la hoja se observa un porcentaje menor de actividad (30.64%). En el caso del ABTS, estos valores de bioaccesibilidad fueron incluso mayores. La muestra original presento valores de (79.17, 78 y 79.33 mgAA/g) y después del proceso de digestión se encontraron valores de 1730.83, 742.91 y 1878.88 mgAA/g para RD, RDA y EH, respectivamente. Dicha actividad antioxidante puede atribuirse a la liberación de compuestos antioxidantes como polifenoles (Ribeiro et al, 2008), Dtocoferoles, carotenoides, fosfolípidos presentes en semilla y cascara del mango (Soong YY et al, 2004; Patricia Morales et al, 2012). Estos compuestos antioxidantes que llegan a sangre aportaran un buen porcentaje de actividad antioxidante. Por otra parte los compuestos no digeridos se asociaron a la fracción indigestible de la muestra, indicándonos que llegan a intestino grueso en donde aporta un ambiente antioxidante. La importancia de este estudio radica en que es la primera contribución de bioaccesibilidad intestinal de los compuestos bioactivos procedentes de los RD, RDA y EH del mango. 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