Agrochimica, Vol. LV - N. 4 July-August 2011 Mineralization and nutrient release of leaf litter and fallen bromeliads in a pine – oak forest in Oaxaca, Mexico C. Roblesa*, M.L. Robles-Martíneza, A. Bautista-Cruza CIIDIR-IPN-Unidad Oaxaca, Laboratorio de Suelos, Calle Hornos 1003, 71230 Santa Cruz Xoxocotlán, Oaxaca, México a Received 28 April 2011 – Received in revised form 07 July 2011 – Accepted 13 July 2011 Keywords: bromeliaceae, leaf litterfall, microcosms, mineralization of organic material, pine-oak forest Introduction. – The species belonging to the family Bromeliaceae, generally refered as bromeliads, are epiphytic plants that are quite common in diverse vegetation types, ranging from semi-arid climates to temperate-humid ones. Their habitual habitat is in the branches of trees and bushes, always tending towards the greatest light incidence (Mondragón et al., 2006). In various regions in Mexico, particularly in the temperate forests of the “Sierra Norte” region of the state of Oaxaca, these plants are harvested from their ecosystems to be sold as decorative objects, and are especially popular as decoration for “nativity scenes”, small, allegorical, homemade structures depicting the birth of Jesus of Nazareth and typically displayed in the Christmas season. Indubitably, this extraction impacts the deposit of organic material and the recycling of nutrients in the ecosystems in which this practice takes place, the importance and relative contribution of which have not been determined. In unaltered conditions, these materials, together with the leaf litter produced by the rest of the plant species which grow in the ecosystem, are mineralized through their fall and decomposition by macro- and microbiota in the soil, thus propitiating the recycling of nutrients which maintains soil productivity in these forests (Bauer and Black 1994). The plant material that falls and dies represents a large percentage of the contribution to the organic material deposited in an ecosystem (Barlos et al., 2007). The positive effects of organic material on soil properties that influence plant development and/or the conservation of those same plant species are widely recognized facts (Bauer and Black 1994, Gallardo et al., 1995, Gressel et al., 1995). Corresponding author: crobles_38@yahoo.it Litter and bromeliads in Oaxaca, Mexico 219 The annual production of deposited leaf litter is highly variable. Its extent depends, among other variables, on the plant species present and the climactic regimen in which they develop. In general, the highest production occurs in ecosystems with warm climates and abundant precipitation, decreasing as temperatures and precipitation descend (RicoGray and Lot 1983, Gallardo et al., 1995, Robles and Barea 2006, Barlow et al., 2007). Very few studies have examined the contribution of bromeliads to the leaf litter deposit in an ecosystem. The seasonality of leaf litterfall is a generalized pattern; however, its point distribution in each ecosystem throughout the year depends on both environmental conditions and the phenology of the plant species present (Riveros and Alberdi 1978, Madeira et al., 1995, Robles and Barea 2006, Barlow et al., 2007, Aceñolaza et al., 2009). The mean decomposition rate for plant material deposited on the ground is also affected by climactic variables, as well as by the soil itself (Isaac and Nair 2005, Goya et al., 2008), due to the composition of the plant material in accordance with the species present (Gallardo et al. 1995, Huang and Schoenau 1997). As with deposit, mineralization rate also depends on environmental conditions, in particular the available moisture in the soil (Gressel et al., 1995) and the chemical composition of the material which undergoes this process (Riveros and Alberdi 1978, Mtambanengwe and Kirchmann 1995, Polyakova and Billor 2007). The objective of this study was to estimate the impact of fallen bromeliads extraction on the dry matter deposit at soil and nutrient cycling in a Pinus-Quercus forest in Santa Catarina Ixtepeji, Oaxaca, Mexico. There are not published data about the magnitude in the reduction of dry matter deposit at forest soil or in nutrient cycling due to this activity. We hypothesize that values of reduction around 1% for both parameters are acceptable to consider sustainable this human activity. Materials and Methods. Study Site. – The study site is located in the Sierra Norte region of the Mexican state of Oaxaca, in the municipality of Santa Catarina Ixtepeji, coordinates 96º33´ longitude West and 17º16´ latitude North (Fig. 1). The altitude at this site ranges from 1,920 to 3,200 m above sea level. The climate is temperate-subhumid, with an average annual temperature of 18.1ºC and an average annual rainfall of 756 mm, concentrated at the end of spring and during summer (Fig. 2) (Serrano-Altamirano et al., 2005). The plant communities in the site are made up of species of the genera Abies, Pinus and Quercus, which are mixed together with one genus being dominant, forming four vegetation types: Quercus-Pinus, Pinus-Quercus, Pinus-Abies, and Abies-Pinus forests. The study sites are located in Pinus-Quercus forests, which is the type of primary vegetation that covers the largest area in the municipality and from which the majority of 220 c. robles et al. Fig. 1. – The Santa Catarina Ixtepeji municipality in Oaxaca, Mexico. Sample sites: El Cerezal (EC) and Ixtepeji (IX). Temperature C° bromeliads are extracted for their subsequent sale. The dominant species in this ecosystem are Pinus pseudostrobus Lindl., P. apulcensis Mart., P. leiophylla Schltdl. & Cham., as well as Quercus crassifolia Humb. & Bonp., Q. acutifolia Nee, Q. rugosa Nee and Q. laurina Humb. & Bonp. (García-Mendoza and Torres-Colín 1999). The average tree density is estimated at 2,200 per hectare, with tree heights ranging from 9 to 15 m. Eight species of bromeliads have been reported for this area: Tillandsia bourgaei Baker, T. prodigiosa Lem. (Baker), T. juncea Ruiz & Pav., T. usneoides L., T. magnusiana Wittm., T. calothyrsus Mez, Catopsis berteroniana (Schult. & Schult. f.) Mez, and Viridantha plumosa Baker (Mondragón et al., 2006). Precipitation mm Fig. 2. – Ombrothermic diagram of Santa Catarina Ixtepeji, Oaxaca, Mexico. Mean data from the period 1961-2003. (Adapted from Serrano-Altamirano et al. 2005). Litter and bromeliads in Oaxaca, Mexico 221 Fallen Bromeliad and Leaf Litter Production. – Five quadrants measuring 10 m by 10 m were marked in each of two sites in the forest with similar floristic composition, locally known as Ixtepeji (IX), at 2,200 m above sea level, with 91% arboreal plant cover, and El Cerezal (EC), at 2,320 m above sea level, with 78% coverage, respectively, separated by a distance of about four kilometers. The quadrants were delineated by perimeter marking. Three collection trays of 0.1 m2 in area and 20 cm in height were placed in each of the quadrants for leaf litter collection (15 trays totaling 1.5 m2 of total collection surface area at each site). The collection of fallen bromeliad individuals was performed by hand, and the total dry weight obtained from the 100 m2 quadrants was recorded. Collection was performed on a monthly basis over a period of 12 months, beginning in August 2006. In the laboratory, the material was cleaned and dried (65°C, 72 h), the dry weight of the material was recorded. All samples were analyzed twice to check the precision of the instrumental methods. Standard reference materials have not been analyzed. The analytical values were accepted only when the data did not differ in any more than 10% between duplicates. The collected data were used to estimate the relative contribution of each type of material to the total soil nutrient pool. The following elements were analyzed with the methods noted in parentheses: nitrogen (microKjeldahl), phosphorus (dry digestion, determination by the molibdovanadate method), calcium and magnesium (atomic absorption spectrophotometry after dry digestion) (Lachica et al., 1973, Kalra 1998). Using the values and concentrations obtained from the dry material, the total nutrient content in the leaf litter and in the fallen bromeliads was estimated for each collection date and site. Bromeliad and Leaf Litter Mineralization. – Microcosms were constructed as an accelerated method for determining the mineralization rate of leaf litter as well as the bromeliad biomass, and the nutrient release from these materials (Day 1983, Wohl and McArthur 2001, Treonis et al., 2002, Bonkowski and Roy 2005, Vargas et al., 2006). For each combination of plant material and site, 5 g of humified organic material taken from the forest were placed in the bottom of 20 Petri dishes (12 cm in diameter). The material had been previously air dried and sifted through a 2 mm screen, which allowed the inoculation of native mineralizing microbiota from the forest floor. This material was then moistened with 25 mL of distilled water, and covered with a screen with 0.5 mm openings. Five g of recently collected leaf litter or bromeliad foliage tissue from each site (dried at 65ºC, 72 h, previously analyzed) was placed on top of the screen. The boxes were closed and sealed with parafilm, then placed in a darkened growth chamber, with a regulated temperature of 18ºC, mimicking the average forest temperature from where the material was collected. Four boxes of each plant material were collected after 15, 30, 45, 60 and 75 days of incubation. The material remaining in the top of the screen was carefully cleaned with a soft brush, dried as cited and its dry weight was recorded. Subsequently, the material was ground and analyzed to determine its nitrogen, phosphorus, calcium and magnesium content, employing the previously mentioned methods. The release percentage of each nutrient was estimated for each combination of plant material type and sampling site for each mineralization timeframe. The mineralization dynamic of each combination of material and sampling site was analyzed using five mathematical models: lineal, logarithmic, potential, exponential and polynomial (quadratic). The fit equation for each model was derived, and variance analysis for each model was carried out; the determination coefficient value (R2) was determined in each case. Statgraphics v. 5.2. software was used for this analysis. 222 c. robles et al. Results. – The magnitude of deposited leaf litter was found to be 3.63 (±0.87) and 4.09 (±0.90) Mg ha-1 year-1 in the EC and IX sites, respectively. Regarding fallen bromeliads, a quantity of 0.046 (±0.01) and 0.021 (±0.006) Mg ha-1 year-1, respectively, was estimated (Tab. 1). The recorded biomass of fallen bromeliad individuals represents a relatively small contribution to the dry material deposited in the ecosystem, 1.27% in EC and 0.52% in IX, in relation to the magnitude of fallen leaf litter. The depositing of leaf litter displayed a seasonal behavior determined by precipitation, with maximum values recorded in the season with the highest environmental temperature and lowest soil humidity, from January to April. In this quarter of the year, 55.6% of the total leaf litter was deposited in the EC site and 75.8% in the IX site. In terms of fallen bromeliads, no clear seasonal behavior was observed; in the EC site, the highest biomass value for these organisms was recorded in December, whereas in the IX site it was recorded in October (Fig. 3). The data recorded in this ecosystem for deposited dry material and its nitrogen, phosphorus, calcium and magnesium content indicates significant amounts of nutrients able to be recycled back into the system through the process of mineralization in the soil. In general, the amounts Table 1. – Bromeliads (B) and leaf litter (H) fall dry weight (g 100 m-2 - standard deviation) throughout one year (2006-2007) in two sites (El Cerezal-EC and Ixtepeji-IX) of pine – oak forest in the Santa Catarina Ixtepeji municipality in Oaxaca, Mexico Month \ site ECB IXB ECH IXH August 54,1 (7,0) 8,0 (2,8) 1991 (677) 162 (21) September 25,2 (5,5) 25,3 (3,0) 2571 (309) 1088 (239) October 7,3 (1,1) 60,7 (11,5) 3998 (760) 1502 (225) November 48,5 (13,1) 24,1 (7,2) 1503 (451) 1754 (474) December 112,3 (23,6) 1,9 (0,4) 1496 (329) 2339 (491) January 50,1 (6,0) 7,6 (2,2) 5233 (1518) 2155 (647) February 52,8 (15,8) 21,3 (3,4) 7261 (1162) 12068 (1448) March 3,6 (0,9) 12,6 (3,4) 5056 (1365) 9569 (2488) April 31,5 (10,4) 18,3 (5,6) 2663 (826) 7229 (2386) May 35,0 (5,3) 12,0 (3,4) 2090 (585) 1685 (253) June 12,6 (6,7) 7,7 (1,6) 1873 (318) 1205 (229) July 29,4 (9,1) 13,8 (3,2) 597 (137) 158 (49) 0,046(0,01) 0,021(0,00) 3,63(0,87) 4,09(0,90) Prod. (Mg ha-1año-1) Litter and bromeliads in Oaxaca, Mexico 223 Litter Fig. 3. – Plant material dry weight of bromeliads (B) and leaf litter (H) fall in two sites of pine – oak forest, El Cerezal (EC) and Ixtepeji (IX), throughout one year (20062007) in the Santa Catarina Ixtepeji municipality, Oaxaca, Mexico. recorded in Kg ha-1 year-1 ranged from 43.6 to 50.6 for nitrogen, from 0.65 to 0.94 for phosphorus, from 138.2 to 231.8 for calcium, and from 28.1 to 29.4 for magnesium. In accordance with the greater magnitude of litterfall in the IX site, the contribution of this site was also found to be greater than that of EC for the four nutrients analyzed in this study. The contribution of fallen bromeliads to the nutritional content of the forest topsoil was, similar to their contribution to deposited dry material, very small, with an average of 0.85% for nitrogen, 0.48% for phosphorus, 0.62% for calcium and 0.60% for magnesium (Tab. 2) with respect to the leaf litter contribution. The mineralization of dry material followed a similar pattern in the four combinations of material type and collection site (Fig. 3). Nevertheless, it was clear that leaf litter was a more recalcitrant material IXH ECH IXB ECB Site \ 0,18 0,09 32,1 3,9 P Ca Mg 9.821 7.207 1.451,4 Ca Mg 10.042 714 78,7 Ca Mg 528,8 11,97 P 1.327 658 1,46 N 1.249,5 136,26 31,86 P 3.137 1.239 N 6,2 101,5 30,9 12,3 9,9 32,9 N Mg 31,0 0,58 0,98 110,9 P 30,7 sep 66,1 aug Ca N nutrient \ sampling month 1.824,9 6.624 21,03 1.832 5.545,2 25.827 43,98 4.878 29,5 243,4 0,43 74,1 3,6 29,3 0,03 8,8 oct 2.131,1 6.472 45,60 2.140 1.182,9 3.081 24,05 1.834 11,71 59,3 0,27 29,4 11,8 136,8 0,10 59,2 nov 1.136,7 8.732 30,41 2.854 727,1 4.832 11,97 1.825 1,6 7,6 0,12 2,3 27,3 316,7 0,34 137,0 dec 29.843 36,31 8.858 15,5 69,0 0,43 26,0 51,3 64,9 1,16 64,4 feb 73.011 313,77 14.723 44.400 248,79 11.674 5.859,9 24.370 161,79 6.168 6,1 49,0 0,11 15,4 1,8 7,0 0,05 4,4 mar 60.796 195,18 8.819 1.294,2 12.836 82,55 3.249 17,8 125,7 0,20 22,3 19,2 78,1 0,35 38,4 apr 8.812 20,22 2.056 925,9 5.037 22,99 2.550 8,8 27,1 0,08 14,8 17,0 183,1 0,32 42,8 may 1.047,3 5.720,2 11.626,3 2.906,1 1.354,7 4.806 28,02 2.629 1.271,6 5.293,3 10.728 62,80 6.384 3,7 30,5 0,11 9,2 12,2 151,3 0,35 61,1 jan 743,5 5.314 13,26 1.470 2.358,1 5.282 20,60 2.285 7,5 18,9 0,09 11,8 15,3 40,7 0,09 15,4 jun 230,4 1.215 1,11 193 725,4 2.907 8,36 728 6,7 55,3 0,15 6,5 14,3 36,2 0,27 11,3 jul 29.328,7 230.940 930,8 50.375 27.884,7 137.011 643,5 43.135 118,9 819,4 2,26 252,6 218,8 1.186,0 4,62 539,6 Total Table 2. – Mean nutrient content (N, P, Ca and Mg - g ha-1) in bromeliads (B) and leaf litter (H) fall throughout one year (2006-2007) in two sites (El Cerezal-EC e Ixtepeji-IX) of pine – oak forest in the Santa Catarina Ixtepeji municipality in Oaxaca, Mexico 224 c. robles et al. Litter and bromeliads in Oaxaca, Mexico 225 Fig. 4. – Microcosm mineralization performance of plant material from bromeliads (B) and leaf litter (H) fall in two sites of pine – oak forest, El Cerezal (EC) and Ixtepeji (IX), throughout one year (2006-2007) in the Santa Catarina Ixtepeji municipality, Oaxaca, Mexico. than bromeliad tissue; additionally, the bromeliad tissue in the IX site was mineralized at a greater rate than that of the EC site. Mathematical modeling of mineralization process behavior indicated that, in all models tested, a significant fit was obtained for the process in all of the combinations of material type and collection site. Nevertheless, the determination coefficient corresponding to the polynomial quadratic model was, in all cases, the highest of the five models tested (Tab. 3). Nutrient release, on average, occurred following a similar dynamic as that of the loss of biomass process. The behavior of the process for leaf litter and bromeliads in the tested system displayed a net release ranging from 29.9% to 53.1% for nitrogen, from 36.4% to 74.7% for phosphorus, from 33.3% to 63.8% for calcium and from 63.6% to 79.2% for magnesium (Tab. 4). The combinations of material type and collection site yielded relatively uniform average values of nutrient release, rounding to 45% for the four nutrients analyzed, with the exception of bromeliads in the IX site, whose average nutrient release value was found to be greater than 67%. Discussion. – The magnitude of deposited leaf litter is affected by various factors, which can be summarized in the type of ecosystem to 2 R Equation 2 R Equation 2 R Equation *P < 0,05, **P < 0,01 Potential Polynomial Exponential 0,947** 0,940* 0,944** 0,931* Y=5,164X Y=5,240X Y=5,206X -0,175 0,994** -0,366 0,959** -0,287 0,963** 2 Y=0,08X -1,03X+6,0 Y=0,008X -0,333X+5,325 0,993** Y=0,065X -0,851X+5,86 2 Y=5,334e Y=5,512e 0,936* 2 0,952** -0,064X Y=-0,759lnX+5,119 0,992** Y=-0,274X+5,247 EC H 0,952** -0,129X Y=-1,374lnX+5,108 0,911* Y=-0,47X+5,26 IX B 0,932* Y=5,416e -0,101X Y=-1,147lnX+5,12 Equation 0,912* R2 R2 Y=-0,939X+5,257 Equation Linear Logarithmic EC B Model \ Site-Plant Mat. 0,947* Y=5,101X-0,168 0,967** Y=0,025X2-0,434X+5,402 0,957** Y=5,231e-0,06X 0,960** Y=-0,728lnX+5,067 0,948* Y=-0,256X+5,165 IX H Table 3. – Mathematical models that explain the mineralization process of dry material from bromeliads (B) and leaf litter (H) fall in two sites of pine – oak forest: El Cerezal (EC) and Ixtepeji (IX) in the Santa Catarina Ixtepeji municipality in Oaxaca, Mexico 226 c. robles et al. Litter and bromeliads in Oaxaca, Mexico 227 Table 4. – Mean nutrient content (N, P, Ca y Mg - mg microcosmos-1) and percent of nutrient release from the residual plant dry material of bromeliads (B) and leaf litter (H) from two sites (El Cerezal-EC and Ixtepeji-IX) of pine – oak forest in the Santa Catarina Ixtepeji municipality in Oaxaca, Mexico, in a microcosm mineralization experimental assay. Site \ nutrient \ days of mineralization ECB IXB ECH IXH 0* 15 30 45 60 75 % release** N 61,3 57,7 45,1 44,8 38,7 38,2 37,7 P 0,55 0,44 0,46 0,44 0,39 0,35 36,4 Ca 130,5 123,6 109,0 102,6 80,5 87,1 33,3 Mg 26,4 12,8 8,9 13,2 8,0 8,6 67,4 N 68,4 55,3 41,2 39,4 36,8 32,1 53,1 P 0,75 0,72 0,36 0,32 0,29 0,19 74,7 Ca 188,5 150,4 88,3 100,4 88,4 68,2 63,8 Mg 30,8 15,5 12,8 8,8 8,8 6,4 79,2 N 71,0 58,6 53,1 50,8 48,0 44,7 37,0 P 0,90 0,57 0,68 0,49 0,42 0,43 52,2 Ca 188,0 180,8 155,6 146,7 135,6 115,9 38,4 Mg 37,0 22,9 20,7 18,3 16,9 11,7 68,4 N 64,9 56,7 51,0 52,6 50,3 45,5 29,9 P 0,80 0,89 0,57 0,51 0,42 0,42 47,5 Ca 267,0 214,8 160,1 184,1 185,9 171,0 36,0 Mg 39,0 63,6 20,5 17,4 17,2 16,2 14,2 * Values of original plant dry matter, sampled before the beginning of the microcosm mineralization experiment. ** Estimated values based in the residual content of each nutrient 75 days after the beginning of the microcosm mineralization experiment. be analyzed. The most relevant ecosystem components are the plant species present, the plant cover or density of individuals, the age of the vegetation, and climate components such as thermal and pluvial regimens (Riveros and Alberdi 1978, Kirman et al., 2007, Pandey et al., 2007, Tateno et al., 2007). Information regarding leaf litterfall in temperate pine forests or pine-broadleaf forests is relatively scarce in comparison with studies of different types of tropical vegetation, whether dry (Robles and Barea 2006) or humid (Rico-Gray and Lot 1983, Rezende et al., 1999, Barlow et al., 2007, Kirman et al., 2007). The magnitude of leaf litterfall found in this study coincides with the results reported by Huber and Oyarzun (1983) for a Pinus radiate D. 228 c. robles et al. Don plantation in the south of Chile, with values fluctuating between 3.7 and 4.13 Mg ha-1 year-1; it is also comparable to that reported by Pandey et al. (2007), namely of 5.48 and 4.20 Mg ha-1 year-1 for a native forest with Quercus serrata Sieb & Zucc. dominance, as well as four associated arboreal species and a plantation of Q. serrata, respectively, in Northeast India. No other reports are found in the literature regarding leaf litter production for the type of vegetation examined in this study. The production of leaf litter in the IX site was close to 13% greater than that of the EC site; this may be attributable to the difference in arboreal cover between the sites, which was 17% greater in the former site than in the latter. This explanation is confirmed by the observation of Donoso et al. (1999), who reported leaf litter production in Drimys winteri Forst. stands to be 27% greater in sites with greater tree density. The leaf litter production contribution of species belonging to the Bromeliacea family has not been widely studied. Riveros and Alberdi (1978), on studying leaf litter production in a hygrophile forest in the south of Chile, reported a very small contribution for the bromeliad Fascicularia bicolor R. et Pav., barely 0.13% of the total deposited leaf litter production throughout the year. This result is less than the results of the present study in either of the two study sites. In the ecosystem examined in the present work, bromeliad species diversity was greater than that for the study conducted by Riveros and Alberdi (1978), which included eight reported species. It is possible that the abundance of the species considered in the present study is also greater than that of Riveros and Alberdi (400 to 650 individuals per hectare in the present study, non-published data), as the aforementioned work does not report the density of F. bicolor individuals in the study site. This greater diversity and abundance of bromeliads may also explain in part the values found in the present study. The seasonal behavior of leaf litterfall is not an inalterable rule. Even considering that it has been reported in many studies (Robles y Barea 2006, Kirman et al., 2007, Pandey et al., 2007, Aceñolaza et al., 2009), there are cases in the literature in which this process does not follow the same behavior (Huber and Oyarzun 1983). The phenological species cycle determines to a great degree the seasonality of litterfall, in conjunction with environmental variables such as soil moisture and temperature. The evidence shows that in this study, these were the determining factors for the seasonality of collected leaf litter, though not for bromeliads, whose fall could be better characterized as accidental in nature. The causal Litter and bromeliads in Oaxaca, Mexico 229 factors for bromeliad fall were wind speed, certain animals such as birds and squirrels which land or stand on the plants or break tree branches, and even humans, who, in the process of collecting forest components (firewood, fruit, etc), accidentally knock bromeliads down. In general, the composition and thus total quantity of nutrients contained in fallen leaf litter is determined by the species present in the ecosystem, the age of the individuals, and the characteristics of the soil itself (Huber et al., 1986, Aceñolaza and Gallardo-Lancho 1994, Gallardo et al., 1995, Mtambanengwe and Kirchman 1995, Tateno et al., 2007). Huber et al. (1986) reported a nitrogen contribution of 27.7, a phosphorus contribution of 3.1, a calcium contribution of 21.3, and a magnesium contribution of 4.4 Mg ha-1 year-1 for a Pinus radiate forest; these results are less than those found in the present study, with the exception of phosphorus, which is considerably higher. It has been reported that pine needles are rich in lignin and phenols, two compounds which slow down the mineralization process of plant material (Scholes and Nowicki 1998). Tateno et al. (2007) found that Robinia pseudoacacia L., an arboreal legume known to fix atmospheric nitrogen, deposits an estimated 56.5 Mg ha-1 year-1 of nitrogen in the form of leaf litter, whereas, in the same system, Quercus liaotungensis Koidz. deposits only 39.1 Mg ha-1 year-1. These quantities are slightly higher and lower, respectively, than the magnitude interval of released nitrogen found in this study. The mineralization rate of leaf litter has been studied by many authors for different ecosystems and the litter bag technique is most commonly used. The study of the process dynamics in systems modeled on microcosms is less common, but it is equally useful for the same purpose, with the advantage of not being subject to probable contamination of the study material, as well as allowing a strict control of the process conditions to be maintained, particularly in terms of moisture content, temperature, light, and microbial population (Day 1983, Vargas et al., 2006, He et al., 2009). Mathematical modeling of the leaf litter mineralization process has found that, depending on the ecosystem being studied, the data can fit either a lineal model (Isaac y Nair 2005, Pandey et al., 2007) or an exponential one (Leblanc et al., 2006, Polyakova y Billor 2007, He et al., 2009). In this study, this process fit to the following five models was analyzed: lineal, logarithmic, exponential, polynomial quadratic and potential. It was found that both leaf litter and bromeliad mineralization fit all of the models significantly, although 230 c. robles et al. the greatest determination coefficients were obtained using the quadratic model. The daily mineralization rate, according to the exponential model, is from 0.06 to 0.064 for leaf litter, and from 0.101 to 0.129 for bromeliads. A rapid mineralization phase is recognizable in the latter material, occurring in the first 30 days, which then changes to a lower rate. The mineralization rate for leaf litter, however, remains relatively constant for at least 75 days. Under the conditions in which the process was performed, there is a net release of nitrogen, phosphorus, calcium and magnesium. This behavior does not occur in the same way when the process occurs in an ecosystem, where the contributions of the soil, of neodeposited materials, or of microbial and root exudates as added factors allow the process of immobilization or gain to occur in a given period (Alvarez et al., 2008). Nutrient release is proportionally higher in bromeliads than in leaf litter, as is weight loss. Even when the mineralization process is recognized as the primary factor responsible for the maintenance of productivity in an ecosystem (Geissen and Morales-Guzmán 2006, Álvarez et al., 2008), the rapid decomposition of deposited dry material may have unfavorable consequences for the soil. Rapid decomposition may impede the formation of topsoil or O horizon that protects the soil from direct solar exposure, and consequently reduces evaporation, as well as offering significant protection from the effects of erosion (Tateno et al., 2007). Despite having a greater nutrient release rate than that determined for leaf litter, the contribution of bromeliads to nutritional recycling in this ecosystem is, as with its contribution to the deposit of dry material, very small: on average, less than 1% for all the nutrients evaluated. Acknowledgements. – The authors would like to thank the Alfredo Harp Helú Foundation for the funding of this study. They would also like to thank the citizens of Santa Catarina Ixtepeji for allowing access to the study sites and permitting the collection of samples. Miss Kimberly Traube revised the English version of the manuscript. REFERENCES Aceñolaza, P.G. and Gallardo-Lancho, J.F.: Pérdida de peso seco en hojarasca de Alnus acuminata en la provincia de Tucumán (Argentina). Bosque, 15, 51-54 (1994). Aceñolaza, P.G., Zamboni, L.P. and Gallardo-Lancho, J.F.: Aporte de hojarasca en bosques del predelta del río Paraná (Argentina). Bosque, 30, 135-145 (2009). Álvarez, E., Fernández-Marcos, M.L., Torrado, V. and Fernández-Sanjurjo, M.J.: Dynamics of macro- Litter and bromeliads in Oaxaca, Mexico 231 nutrients during the first stages of litter decomposition from forest species in a temperate area (Galicia, NW Spain). Nutr. Cycl. Agroecosyst., 80, 243-256 (2008). Barlow, J., Gardner, T.A., Ferreira, L.V. and Peres, C.A.: Litter fall and decomposition in primary, secondary and plantation forests in the Brazilian Amazon. For. Ecol. Manag., 247, 91-97 (2007). Bauer, A. and Black, A.L.: Quantification of the effect of soil organic matter content on soil productivity. Soil Sci. Soc. Am. J., 58, 185-193 (1994). Bonkowski, M. and Roy, J.: Soil microbial diversity and soil functioning affect competition among grasses in experimental microcosms. Oecologia, 143, 232-240 (2005). Clark, K.L., Nadkarni, N.M., Schaefer, D. and Gholz, H.L.: Atmospheric deposition and net retention of ions by the canopy in a tropical montane forest, Monteverde, Costa Rica. J. Trop. Ecol., 14, 27-45 (1998). Day Jr., F.P.: Effects of flooding on leaf litter decomposition in microcosms. Oecologia, 56, 180-184 (1983). Donoso, C., Maureira, C., Zuñiga, A. and Castro, H.: Producción de semillas y hojarasca en renovales de canelo (Drimys winteri Forst.) en la Cordillera de la Costa de Valdivia, Chile. Bosque, 20, 65-78 (1999). Gallardo, J.F., Santa Regina, I., Harrison, A.F. and Howard, D.M.: Organic matter and nutrient dynamics in three ecosystems of the “Sierra de Bejar” mountains (Salamanca province, Spain). Acta Oecologica, 16, 447-459 (1995). García-Mendoza, A. and Torres-Colín, R.: Estado Actual del Conocimiento sobre la Flora de Oaxaca. Vegetación y Flora. Sociedad y Naturaleza en Oaxaca 3. Instituto Tecnológico Agropecuario de Oaxaca, Oaxaca (México), pp. 49-86 (1999). Geissen, V. and Morales-Guzmán, G.: Fertility of tropical soils under different land use systems – a case study of soils in Tabasco, Mexico. Appl. Soil Ecol., 31, 169-178 (2006). Gressel, N., McColl, J.G., Powers, R.F. and McGrath, A.E.: Spectroscopy of aqueous extracts of forest litter. II. Effects of management practices. Soil Sci. Soc. Am. J., 59, 1723-1731 (1995). Goya, J.F., Frangi, J.L., Pérez, C. and Dalla-Tea, F.: Decomposition and nutrient release from leaf litter in Eucalyptus grandis plantations on three different soils in Entre Ríos, Argentina. Bosque, 29, 217-226 (2008). He, X., Zhang, P., Lin, Y., Li, A., Tian, X. and Zhang, Q.H.: Responses of litter decomposition to temperature along a chronosequence of tropical montane rainforest in a microcosm experiment. Ecol. Res., 24, 781-789 (2009). Huang, W.Z. and Schoenau, J.J.: Mass loss measurements and statistical models to predict decomposition of leaf litter in a boreal aspen forest. Commun. Soil Sci. Plant Anal., 28, 863-874 (1997). Huber, A. and Oyarzun, C.: Producción de hojarasca y su relación con factores meteorológico en un bosque de Pinus radiata (D.Don.). Bosque, 5, 1-11 (1983). Huber, A., Schlatter, J.E. and Oyarzun, C.: Aporte en elementos nutritivos por la hojarasca de un bosque adulto de Pinus radiata. Bosque, 7, 59-64 (1986). Isaac, S.R. and Nair, M.A. Biodegradation of leaf litter in the warm humid tropics of Kerala, India. Soil Biol. Biochem., 37, 1656-1664 (2005). Kalra, Y.P. (Ed.): Handbook of reference methods for plant analysis. Soil and Plant Analysis Council, Inc. CRC Press. New York, USA (1998). Kirman, S., Strasbeerg, D., Grondin, V., Colin, F., Gilles, J. and Meunier, J.D.: Biomass in litterfall in a native lowland rainforest: Marelongue Reserve, La Réunion Island, Indian Ocean. For. Ecol. Managem., 252, 257-266 (2007). Lachica, M, Aguilar, A. and Yánez, J.: Análisis foliar. Métodos utilizados en la Estación Experimental del Zaidín. Anal. Edafol. Agrobiol., 32, 1033-1047 (1973). Leblanc, H.A., Nygren, P. and McGraw, R.L.: Green mulch decomposition and nitrogen release from leaves of two Inga spp. in an organic alley-cropping practice in the humid tropics. Soil Biol. Biochem., 38, 349358 (2006). Madeira, M., Araújo, M.C. and Pereira, J.S.: Effects of water and nutrient supply on amount and on nutrient concentration of litterfall and forest floor litter in Eucalyptus globulus plantations. Plant Soil, 168-169, 287-295 (1995). Mondragón, D.M., Ramírez, I.M.M., Villa, D.M.G., Escobedo, G.J.S. and Franco, A.D.F.: La riqueza de bromelias epífitas a lo largo de un gradiente altitudinal en Santa Catarina Ixtepeji, Oaxaca, México. Natur. Des., 4, 13-16 (2006). Mtambanengwe, F. and Kirchmann, H.: Litter from a tropical savanna woodland (Miombo): chemical composition and C and N mineralization. Soil Biol. Biochem., 27, 1639-1651 (1995). Pandey, R.R., Sharma, G., Tripathi, S.K. and Singh, A.K.: Litterfall, litter decomposition and nutrient dynamics in a subtropical natural oak forest and managed plantation in northeastern India. For. Ecol Managem., 240, 96-104 (2007). Polyakova, O. and Billor, N.: Impact of deciduous tree species on litterfall quality, decomposition rates and nutrient circulation in pine stands. For. Ecol. Managem., 253, 11-18 (2007). Rezende, C.P., Cantarutti, R.B., Braga, J.M., Gomide, J.A., Pereira, J.M., Ferreira, E., Tarré, R., Macedo, R., Alves, B.J.R., Urquiaga, S., Cadisch, G., Giller, K.E. and Boddey, R.M.: Litter deposi- 232 c. robles et al. tion and disappearance in Brachiaria pastures in the Atlantic forest region of the South of Bahia, Brazil. Nutr. Cycl. Agoecosyst., 54, 99-112 (1999). Riveros, M. and Alberdi, M.: Acumulación de hojarasca en un bosque de olivillo (Aextoxicon punctatum R. et Pav.) del fundo San Martín (Valdivia, Chile). Bosque, 2, 72-82 (1978). Rico-Gray, V. and Lot, A.: Producción de hojarasca del manglar de la Laguna de la Mancha, Veracruz, México. Biotica, 8, 295-301 (1983). Robles, C. and Barea, J.M.: Deposición de hojarasca y reciclamiento de nutrientes en un ecosistema mediterráneo. Memoria del II Congreso Ibérico de la Ciencia del Suelo. Huelva, España, (2006). Scholes, M.C., and Nowicki, T.E.: Effects of pines on soil properties and processes. In: Ecology and Biogeography of Pinus (Richardson, D.M., ed.). Cambridge University Press. Cambridge. pp. 341-353 (1998). Serrano-Altamirano, V., Silva-Serna, M.M., Cano-García, M.A., Medina-García, G. and Ruiz-Corral, A.: Estadísticas climatológicas básicas del estado de Oaxaca (periodo 1961-2003). INIFAP-SAGARPA. Oaxaca, México, (2005). Tateno, R., Tokuchi, N., Yamanaka, N., Du, S., Otsuki, K., Shimamura, T., Xue, Z., Wang, S. and Hou, Q.: Comparison of litterfall production and leaf litter decomposition between an exotic black locust and an indigenous oak forest near Yan´an on the Loess Plateau, China. For. Ecol. Managem., 241, 84-90 (2007). Treonis, A.M., Wall, D.H. and Virginia, R.A.: Field and microcosm studies of decomposition and soil biota in a cold desert soil. Ecosystems, 5, 159-170 (2002). Vargas, D.N., Bertiller, M.B., Ares, J.O., Carrera, A.L. and Sain. C.L.: C and N dynamics induced by leaf-litter decomposition of shrub and perennial grasses of the Patagonian Monte. Soil Biol. Biochem., 38, 2401-2410 (2006). Whol, D.L., and McArthur, J.V.: Aquatic actinomycete-fungal interactions and their effects on organic matter decomposition: a microcosm study. Microbial Ecol., 42, 446-457 (2001). Summary. – The aim of the work was to estimate the impact of fallen bromeliads extraction on the dry matter deposit at soil and nutrient cycling in a pine-oak forest. Leaf litter and fallen bromeliads were collected over a period of 12 months from two sites in the forest, and their dry weight as well as their nitrogen, phosphorus, calcium and magnesium content were recorded. In microcosms, the mineralization rate of each type of material from both sites was analyzed. Leaf litterfall rates were found to be 3.63 and 4.09 Mg ha-1 y-1, and bromeliad litterfall rates were found to be 0.046 and 0.021 Mg ha-1 y-1; the latter represent 1.27% and 0.52%, respectively, of leaf litterfall. The leaf litterfall dynamic showed seasonal behavior; the bromeliad litterfall dynamic did not. The contribution of fallen bromeliads to the nutrient deposit on the forest floor was 0.85% for nitrogen, 0.48% for phosphorus, 0.62% for calcium and 0.60% for magnesium, relative to the leaf litter contribution. Leaf litter was found to be more recalcitrant to mineralization than bromeliads.