SUBJECT: MORPHOLOGY AND FUNCTION OF THE MALE

 MSc in Biology and Technology of Reproduction in Mammals SUBJECT: MORPHOLOGY AND FUNCTION OF THE MALE
REPRODUCTIVE ORGANS (Compulsory subject)
Subject coordinated by: Dr. Octavio López Albors
Anatomy Cellular biology Physiology Students work Anat/Cell Biol/Physiol Begin: 28th September - End: 13th October
1st WEEK
Morning
MONDAY 28
TUESDAY 29
10:30
Lecture room
General
introduction to the
master
9:30 Practice.
Dissection room
Comparative
anatomy of the
pelvis and the male
reproductive organs
in domestic
mammals (dog
model).
(Drs. G Ramírez &
MªD Ayala)
General overview
of the coordinated
subjects
(Drs. R. Romar &
O López)
WEDNESDAY
30
UNIVERSITY
ACADEMIC
YEAR
THURSDAY 1
10:00 Theory &
Practice
Cel Biol Med
Practice Room
Cell Biol Med
(hall 2 floor)
Comparative
sperm
morphology
(Dr. M Avilés)
FRIDAY 2
9:30 Theory
Cel Biol Med
Spermiogenesis
(Dr. M Avilés)
Sperm
morphology
(Dr. M Avilés)
OFFICIAL
12:30 Theory.
Anatomy of the
pelvis and general
patterns of blood
and nerve supply
to the
reproductive
organs
(Dr. O. López)
12:00 Theory.
Lecture room
OPENING
Testicle
physiology
(Dr. J Gadea)
Break
Afternoon
16:00 Theory
Lecture room
Comparative
anatomy of the
male reproductive
organs in
domestic
mammals
(Dr. O López)
16:00 Practice
Dissection room
Comparative
anatomy of the
male reproductive
organs in domestic
mammals (equine,
ruminants, swine
and rodents)
Dissection of fresh
genital organs
(ruminants and pig)
(Drs. G Ramírez,
MªD Ayala & R
Latorre)
16:00 Theory.
Cel Biol Med
Practice Room
Testicle I:
histology
(Dr. JF Madrid)
18:00 Theory.
Cel Biol Med
Practice Room
Testicle II:
spermatogenesis,
sperm ducts and
accessory
reproductive
glands
(Dr. JF Madrid)
MSc in Biology and Technology of Reproduction in Mammals MONDAY 5
9:30 Practice
Cel Biol Med
Practice Room
Cel Biol Med
Testicle and
epididymis
(light microscopy)
(Dra. C.Ferrer y JF
Madrid)
TUESDAY 6
WEDNESDAY 7
8:00 Practice
Veterinary farm
Ejaculate obtaining
in Buck
(Drs. C. Matas & J
Gadea)
8:00 Practice
Veterinary farm
Ejaculate
obtaining in pig
(Drs. C. Matas & J
Gadea)
THURSDAY 8
9:30 Theory
Lecture Room
Physiology of the
epididymis and
reproductive
glands
(Dra. C Matas)
FRIDAY 9
VETERINARY
FACULTY
Puberty and sexual
behaviour
(Dra. C Matas)
OFFICIAL
Morning
Practice.
Electron microscopy
of testicle, spermatic
ducts and accessory
glands)
11:30 Theory
Lecture Room
Scientific
Bibliography
Search
(Dr. J Gadea)
16:00 Lecture room
Student’s selection
of seminars and
topics for
presentations
(Dr. O. López)
16:00 Practice
Lab Physiol
Seminars work
Break
Afternoon
Epididymal fluid
obtaining in pig and
bull
(Dr. C Matas)
Seminars work
HOLLIDAY
MSc in Biology and Technology of Reproduction in Mammals 3rd WEEK
Morning MONDAY 12 TUESDAY 13 09:30 Theory.
Lecture room
Anatomy of the male
reproductive organs (human).
Dr. K de Jong)
NATIONAL
HOLLIDAY
Seminars work Break Afternoon 16:00 Practice
Dissection room
Anatomy of the male
reproductive organs (human).
(Dr. K de Jong)
SEMINARS
1. Sperm maturation (Dr. M Avilés)
2. Testicle thermoregulation: anatomical and physiological aspects (Drs. O López, J. Gadea)
3. Seminal plasma composition (Dra. C. Matas)
4. Erection: anatomy and physiology (Drs. O. López, J Gadea)
5. Ejaculation: anatomy and physiology (Drs. O. López, J Gadea)
6. Regionalization of the sperm membrane (Dr. M Avilés)
7. Hypophysis: comparative anatomy and histology (Drs. O. López, Juan Fº Madrid)
PAPER FOR ROUND TABLE DISCUSSION:
Harrison and Weiner, 1949. Vascular patterns of the mammalian testis and their functional significance
MSc in Biology and Technology of Reproduction in Mammals SUBJECT: MORPHOLOGY AND FUNCTION OF THE FEMALE
REPRODUCTIVE ORGANS (Compulsory subject)
Subject coordinated by: Juan Francisco Madrid
Anatomy Cellularbiology Physiology Studentswork Anat/CellBiol/Physiol Begin: 14th October
End: 23st October
1st WEEK
Morning WEDNESDAY 14 THURSDAY 15 09:30
Lecture room
09:30 Theory.
Lecture room
General overview of the
subject
Anatomy of the female reproductive system (human) (Dr. K. de Jong). 10:00 Theory: Lecture
room
Comparative anatomy of
reproductive organs in
domestic mammals
(Dr. R. Latorre)
FRIDAY 16 9:30 Practice. Dissection room Comparative anatomy of
reproductive organs in
domestic mammals: mare,
cow, sow, rabbit and rat.
(Drs. G. Ramírez & O. López) Seminars work Seminars work 16:00 Practice Lecture
room
16:00 Practice
Dissection room
Anatomy of the female reproductive system (human). (Dr. K. de Jong) Seminars work 12:00 Theory: Lecture
room
Comparative anatomy of
reproductive organs in
domestic mammals
(Dr. R. Latorre)
Break Afternoon Topography of the female reproductive organs. Canine model. (Dr. G. Ramírez & R. Latorre) Seminars choice MSc in Biology and Technology of Reproduction in Mammals 2nd WEEK
Morning MONDAY 19 9:30 Theory. Medicine faculty The ovary histological organization (Dr. M. Avilés) TUESDAY 20 9:30 Practice Faculty of Medicine Microscopic observation of ovary (newborn, Young, menopausal primate, mare) (Dr C. Ferrer) WEDNESDAY 21 9:30 Theory. Lecture room Physiology of female reproductive system. Dr. P Coy 11:30 Estrous cycle in domestic females Dr. R Romar THURSDAY 22 9:30 Practice Faculty of Medicine. Microscopy observation of uterus, oviduct, etc (Dr. J.F. Madrid) FRIDAY 23 9:30 Theory Lecture room Control of estral cycle in domestical animals with special focus in cow Dr. D Rizos 11:30. Menstrual cycle in women. Control of menstrual cycle in women Dra. MT Prieto Break Afternoon 16:00 Theory Medicine faculty Folliculogenesis, oogenesis and female gamete. (Dr. M. Avilés) 16:00 Theory Faculty of Medicine Origin of the zona pellucida (Dr. M. Jiménez) 17:00 Theory Faculty of Medicine Histological organization of the reproductive tract (Dr. C. Ferrer) 15.30 Practice Lab Physiolo Oestrus cycle in the bitch. Dr. P Coy 15:30 Theory Faculty of Medicine Development of the zona pellucida (Dr. M. Jimémez) 16:30 Practice Faculty of Medicine. Electron microscopy of the female reproductive organs MJM (Dr. M. Jiménez) 15.30 Practice Lab Physiol Identification of the different phases of the estrous cycle by observing the ovary Oviduct dissection and culture of oviductal cells Dr. R Romar SEMINARS
1. Composition of the uterine fluid. P Coy.
2. Functional relationship between the ovarian artery and vein: Anatomy
3. Comparative anatomy of the female tract related to the artificial insemination: Anatomy
4. Gene expression in the vagina: M. Avilés
5. Follicular atresia: M.Avilés
MSc in Biology and Technology of Reproduction in Mammals 6.
7.
8.
9.
10.
The polycystic ovarian syndrome in women: MJ Izquierdo,
Clinical cases related with the control of estrous cycle in pets P Coy
Hormonal regulation of the menstrual cycle and its relationship with contraceptives: R.Romar
Seasonal reproductive changes in mare and small ruminants. R Romar
Differential gene expression in the oocyte and cumulus cells depending of the hormone treatment in human:
M Avilés
PAPER FOR ROUND TABLE DISCUSSION:
Shuai Chen, Ralf Einspanier and Jennifer Schoen. In vitro mimicking of estrous cycle stages in porcine oviduct
epithelial cells: Estradiol and progesterone regulate differentiation, gene expression, and cellular function. Biology of
Reproduction 2013, 89:54, 1-12 (Rakel)
CLOSING-OPENING CEREMONY OF MASTER “BIOLOGY AND TECHNOLOGY OF
REPRODUCTION IN MAMMALS” 2014-15 - 2015-16.
Date: 26th October 2015
Place: Graduation Hall, Faculty of Veterinary, University Campus of Espinardo, Murcia, Spain.
Program:
9.30h
Welcome.
Dr. Antonio Rouco. Dean of the Faculty of Veterinary.
Dr. Pilar Garrido Clemente. General Coordinator of Campus Mare Nostrum.
10.00h
Inaugural conference “Advances in techniques to improve fertility of boar
spermatozoa” by Dr. Hiroaki Funahashi. Department of Animal Science Graduate
School of Environmental and Life Science, Okayama University, Japan.
11.00h
Conference “Classification and embryo selection in assisted reproduction
programs” by Dr. Victoria Hurtado de Mendoza. Chair of the Interest Group of
Embryology in ASEBIR (Association for the Study of Reproductive Biology)
12.00h
Coffee
12.30h
Farewell to the XI class of students (course 2014-15) and awarding of diplomas.
Welcome to the students of the course 2015-16.
13.30h
Lunch.
15:00h
Conference “Calcium signaling and oocyte activation” by Dr. John Parrington.
Department of Pharmacology, University of Oxford, United Kingdom.
Contributors:
MScinBiologyandTechnologyofReproductioninMammals
COMMONACTIVITIESOFINTEGRATEDMORPHOLOGY&FUNCTION
SUBJECTS
•
•
•
MORPHOLOGYANDFUNCTIONOFTHEMALEREPRODUCTIVEORGANS
MORPHOLOGYANDFUNCTIONOFTHEFEMALEREPRODUCTIVEORGANS
FERTILIZATION,EMBRYODEVELOPMENTANDPREGNANCY
Begin: 27 October 2015
End: 30 October 2015
MONDAY
26
TUESDAY 27
WEDNESDAY 28
8:45 h. Lecture Room
9:00 h. Lecture Room
“Morphol & Function
of FEMALE organs”
“Morphol &
Function of MALE
organs”
Master
Closing
Morning
•
Discussion of
Scientific Paper
(J. Gadea)
2014-15
//
Opening
2015-16
Journey
Break
15:30 h. Lecture room
Seminars defense:
Afternoon
Nº 1-7
J. Gadea
M. Avilés
O. López
THURSDAY 29
9:30 h. Lecture Room
“Morphol & Function
of FEMALE organs”
Discussion of
Scientific Paper
(R. Romar)
• Seminars defense:
Nº 1,4, 5, 7 y 10
P. Coy
M. Avilés
• Seminars defense:
Nº 2, 3, 6, 8 y 9:
R. Romar
O. López
• Mock Exam:
“MALE and FEMALE
Subjects”
•
FRIDAY 30
GAMETES-OVIDUCT INTERACTIONS BEFORE FERTILIZATION
(1 week/3 ECTS)
Coordinator: Pilar Coy Fuster
Theoretical Sessions: Room -1.3. Library (Veterinary Faculty). Practical Sessions: Department of Veterinary Physiology (1st
floor, Faculty of Veterinary). Optional practical sessions: Veterinary Farm. Research building.
Morning
MONDAY 2nd
TUESDAY 3rd
Nov
Nov
9:00: Theoretical 9:30 Practice
session
II (optional)
Surgical
Presentation.
approach to
Introduction to the the pig oviduct
Subject
for
zygote
P Coy and R
collection.
Romar
WEDNESDAY 4th
Nov
9:00 Practice III
Sperm binding to
oviductal
epithelial cells.
P Coy and R Romar
9:30 Theoretical
session
Generalities of the
oviduct and
oviductal fluid
Interaction
spermatozoa- oviduct.
Sperm reservoir and
capacitation in the
oviduct
R López-Úbeda.
Oviductal
mechanisms
regulating spermoocyte interaction
R Romar
Break
Afternoon
16:00 Practice I.
Obtaining
and
storing oviductal
fluid
P Coy and R
Romar
16:30.
Conference
about
Employment
strategies,
organized by
COIE
THURSDAY
5th Nov
12:00
Theoretical session
Practice IV.
Preparation of
oviductal
fluid samples for
enzymatic
assays.
R Romar
FRIDAY 6th Nov
9:30: Practice VI
Assessment of
the results of the
enzymatic
analysis in
oviductal fluid
R. Romar
12.00
Theoretical
session
Gene expression of
the oviduct and
protein composition
M. Avilés (lecture
at the Medical
school)
16:30 Practice
V
Changes in
zona pellucida
digestion
mediated by
the oviduct
P Coy
SUBJECT: FERTILIZATION, EMBRYO DEVELOPMENT AND PREGNANCY
(Compulsory subject)
From 9th to 20th November 2015
Subject coordinated by P Coy
Lecture room (theoretical sessions): room number-1.3 (located at ground floor of the library of the Veterinary Faculty).
Lab Veterinary Physiology: Department of Physiology, 1st floor Veterinary Faculty. Dissection room (Veterinary
Anatomy).
1st week
MONDAY 9
9:00 h.
Introduction to the
subject
P Coy
TUESDAY 10
Break
Afternoon
THURSDAY 12
8:45 Practice
Lab Histology
Department
Medical School
FRIDAY 13
9:00 h. Theoretical
session
Implantation
and
placentation
Practice
Practice
F. Gil
Obtaining of tubal Obtaining of 2oocytes
cell embryos
9:15 Theoretical
session
Fertilization.
Gamete recognition
María Jiménez
Morning
WEDNESDAY 11
8:45
Lab
Histology
Department
Medical School
M. Avilés
M. Avilés
11:00 h Selection
of seminars
12:30
h.
Theoretical
session
Fertilization.
Acrosomal reaction
and sperm
penetration
MJ Izquierdo
11:30 Theoretical 12:00 h
session
16:00 h Theoretical
session
Fertilization. Fusion
of gametes and the
polyspermy block
M. Avilés
16:00
h
Theoretical
session
Early stages of
embryonic
development (from
zygote
to
blastocyst)
Roles of oviductal
proteins
in
mammalian
fertilization
and
embryo
development
P Coy
F. Gil
12:00 h
12:00 h
2nd WEEK
Morning
MONDAY 16 Nov
9:30 h Theoretical
session
Physiology
of
pregnancy
in
domestic animals P
Coy
TUESDAY 17 Nov
9:00 h Practice:
Dissection room
Comparative
anatomy
of
placentation (I)
F. Gil
11:00 h
O. López
Theoretical session
Fetus development
in humans.
MT Prieto
12:00 h
Break
Afternoon
16:00 h Practice:
Dissection room
Comparative
anatomy
of
placentation (II)
F. Gil
G Ramírez
R Latorre
WEDNESDAY 18
9:30 h Theoretical
session
Physiology of
parturition
R Romar
11:00 h
Theoretical
session
Physiology of
lactation in
domestic animals
R Romar
15:30 Theory
Lecture room
Human anatomy of
male reproductive
organs
Kees de Jong
17:00: Practices
Dissection room
Human anatomy of
male reproductive
organs
THURSDAY 19
11:00 h
Theoretical
session
Main breeds and
reproductive
programation in
small ruminants
herds
C. Gonzalo
Abascal.
12:30 h
Theoretical
session
Lactation curves,
mammary health
and optimization of
production in small
ruminants.
C. Gonzalo
Abascal.
FRIDAY 20
9:30 Theory
Lecture room
Human anatomy of
female reproductive
organs
Kees de Jong
11:00: Practices
Dissection room
Human anatomy of
female reproductive
organs
Kees de Jong
16:00
h.
Theoretical
session
Embryology
of
reproductive
organs
and
mamary gland
F Gil
Kees de Jong
SEMINARIES
1. Sperm competition M Avilés.
2. Species-specific gamete recognition. M. Avilés
3. Teratogens in women. MJ Izquierdo
4. Common malformations in the male reproductive organs. F Gil
5. Common malformations in the female reproductive organs. F Gil
6. Ectopic pregnancy in primates. P Coy
7. Parturition in the mare. R Romar
8. Practical information on lactation in human. R Romar
9. Comparative chronology of fetal development in human and domestic species. P Coy
PAPERS IN DISCUSSION
Juno is the egg Izumo receptor and is essential for mammalian fertilization. Enrica Bianchi, Brendan
Doe, David Goulding ,Sanger Mouse Genetics Project and Gavin J. Wright. Nature. 2014; 508(7497):
483–487. doi:10.1038/nature13203.
Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Haruhiko
Miyata, Yuhkoh Satouh, Daisuke Mashiko, Masanaga Muto, Kaori Nozawa, Kogiku Shiba, Yoshitaka
Fujihara, Ayako Isotani, Kazuo Inaba, Masahito Ikawa.
Sciencexpress/sciencemag.org/content/early/recent/ 1 October 2015 / Page 3/ 10.1126/science.aad0836
MScinBiologyandTechnologyofReproductioninMammals
COMMONACTIVITIESOFINTEGRATEDMORPHOLOGY&FUNCTION
SUBJECTS
•
•
•
MORPHOLOGYANDFUNCTIONOFTHEMALEREPRODUCTIVEORGANS
MORPHOLOGYANDFUNCTIONOFTHEFEMALEREPRODUCTIVEORGANS
FERTILIZATION,EMBRYODEVELOPMENTANDPREGNANCY
Begin: 23 November 2015
End: 27 November 2015
MONDAY 23
9:30 h. Lecture Room
Subject:
“Fertilization,
embryo development
and pregnancy”
Morning
DISCUSSION OF
SCIENTIFIC PAPER
M Avilés
P Coy
SEMINARS
DEFENSE: 1-5
M. Avilés
MJ Izquierdo
F Gil
TUESDAY
24
WEDNESDAY 25
THURSDAY 26
10:00 h. Lecture
Room
Subject:
“Fertilization,
embryo
development and
pregnancy”
9:30: Lecture
room
SEMINARS
DEFENSE: 6-9
P. Coy
R. Romar
MOCK EXAM:
“Fertilization, embryo
development and
pregnancy”
INVITED
SPEAKERS
Dr. Pablo
Bermejo,
INIA, Madrid
“Gene
expression in
the embryo”
Dr. Serafín,
INIA, Madrid
“Sperm sees it
hot: opsins,
the sperm
eyes”
Dr. Pedro L.
Lorenzo UCM,
Madrid
“Immunocontraception”
Break
16:00 Lecture
Room
Afternoon
INVITED SPEAKER:
Mª José Gómez
Torres
“Fetal interactions:
extraembrionary
tissues and
placenta”
15:30 Lecture
room
TUTORIAL:
Questions
about how to
prepare the
topic?
TUTORIAL:
Presentation of
Topics for the
Master
Dissertation
(TFMs)
FRIDAY 27
9:00: Lecture
room
INVITED
SPEAKER:
Dr. F. Sánchez
Margallo
(CCMIJesúsUsónCác
eres, Spain).
“Minimally
invasive surgery
in the female
reproductive
organs”
Time table: SPERM FUNCTIONAL ANALYSIS (2015‐16)
Monday 30
Thuesday 1
Wednesday 2
Thursday 3
Friday 4
Presentation
T. Seminal analysis (Dra.
C. Matas)
T. Sperm capacitation II
(Prof. Visconti)
T. Techniques to evaluate
sperm capacitation
(Dra López‐Úbeda)
T.
Change
in
metabolic
pathways during
capacitation
p
((Prof.
Visconti)
Research Prof. Visconti
T. Sperm capacitation I
(Prof. Visconti)
T. Techniques that assess sperm membrane function . Introduction to fluorescence techniques (microscopy and flow cytometry) (Dr. Gadea)
P : Protein phosphorilation analyzed by IFI and Acrosome
reaction I (Dra. R. López / C. Matas)
T. Prediction of
fertility
(Dr.
Gadea)
T. Study of sperm fertility
by in vitro tehcnique (Dr.
Gadea)
P: Protein phosphorilation
analyzed by IFI
and
Acrosome reaction II (Dra.
R. López / C. Matas)
P.
Routine
semen
evaluation (Dra. Matás/ C.
Soriano)
T. Seminal analysis in
human (Dra. E. Sellés)
P.
Cytometry/
CASA
C
S
(Dr.
Gadea/C.
Soriano)
P.
Citometria/
CASA (Dr. Gadea/
C. Soriano)
P. Evaluation samples
from
fluorescence
techniques
(Dra. Matas)
Lessons
esso s sstart
a aat 9.30. The
9 30
ep
place for
ace o theoretical
eo e ca sess
session
o ((T) will
)
be ‐1.3 room. The
3 oo
ep
place for
ace o p
practice
ac ce sess
session
o ((P) will
)
be
in SACE and physiology lab. Topic: Sperm populations evaluation and CASA system to evaluated motility (Dr. Soler) will be explain the next week)
SUBJECT “IN VITRO MATURATION, IN VITRO FERTILIZATION AND EMBRYO CULTURE”. 9th-22nd December 2015.
Master Course “Biology and Technology of Reproduction in Mammals”. 2015-16.
Theoretical Sessions: Room -1.3. Library (Veterinary Faculty). Practical Sessions: Department of Veterinary Physiology (1st floor, Faculty of Veterinary).
MONDAY 7 Dec
HOLIDAY
TUESDAY 8 Dec
HOLIDAY
WEDNESDAY 9 Dec
9.00h. Presentation. Working groups
arrangement.
Five A-groups: 4 students/group
Seven B-groups: 3 students/group
Three C-groups: 7 students/group
R. Romar
9.30h Lesson 1.
Composition and preparation of culture
media.
S. Cánovas
11.30h. Practice 1.
Groups A1 (11.30h),A2 (12.15h) and A3
(13.00h)
Preparation of culture media for
manipulation of oocytes.
JA. Carvajal and S. Cánovas
HOLIDAY
HOLIDAY
THURSDAY 10 Dec
FRIDAY 11 Dec
9.00h. Lessons 1.
In vitro maturation I (IVM): generalities.
In vitro maturation II (IVM): current
status and results in different
mammalian species.
R. Romar
9:30 Lesson 2.
In vitro maturation in humans.
G. Arroyo. Dexeus Institute,
(Barcelona).
12.00h. Practice 2. (All groups).
Obtaining of pig oocytes. Manipulation
and in vitro maturation.
S. Cánovas and C. Soriano
15.30h. Practice 3. (All groups).
15.00h. Practice 1 (cont.).
Staining and fixing of oocytes.
Groups A4 (15.00h) and A5 (15.45h).
Preparation of culture media for C. Soriano and R.Romar
manipulation of oocytes.
JA. Carvajal and S. Cánovas
11.30h. Lesson 3.
IVM results evaluation.
S. Cánovas
13.00h. Practice 4.
Groups B1 and B2.
Microscopic
evaluation
of
immature and mature oocytes.
C. Soriano and P. Coy
16.00h.
Sperm analysis by CASA
Carles Soler, Proiser R+D SL
(Valencia)
SUBJECT “IN VITRO MATURATION, IN VITRO FERTILIZATION AND EMBRYO CULTURE”. 9th-22nd December 2015.
Master Course “Biology and Technology of Reproduction in Mammals”. 2015-16.
Theoretical Sessions: Room -1.3. Library (Veterinary Faculty). Practical Sessions: Department of Veterinary Physiology (1st floor, Faculty of Veterinary).
MONDAY 14 Dec
9.00h. Lesson 4.
In vitro fertilization I (IVF):
generalities and current status.
In vitro fertilization II (IVF):
evaluation of results after IVF C.
Matas
TUESDAY 15 Dec
THURSDAY 17 Dec
FRIDAY 18 Dec
9.30h. Practice 5.
Group C1.
Porcine IVF.
C. Matas
9.30h. Practice 6.
Groups B6 and B7.
Microscopic evaluation of zygotes.
R. Romar and S. Cánovas
9.00h. Lesson 5.
Intracitoplasmic sperm injection
(ICSI).
FA. García
9.00h. Practice 7 (cont.).
Group B4.
ICSI of pig oocytes
FA. García
11.00h. Practice 5 (cont.).
Group C2.
Porcine IVF.
C. Matas
11.00h. Practice 6 (cont.).
Groups B4 and B5.
Microscopic evaluation of zygotes.
C. Matas and C. Soriano
11.00h. Practice 7.
Group B1.
ICSI of pig oocytes
FA. García
11:00h Doctoral Thesis
(Veterinary Farm)
Title: Afinamiento de la zona
pelúcida mediante laser
(AZPL) en ciclos de FIV/ICSI
en 2 grupos de edad y su
relación con el grosor de la
zona pelúcida”.
B. Amorocho (IVI-Murcia)
12.00h. Practice 4 (cont.).
Groups B5 and B6.
Microscopic
evaluation
of
12.30h. Practice 5 (cont.).
oocytes (immature and mature)
Group C3.
C. Soriano and R. Romar
Porcine IVF.
C. Matas
13.30h. Practice 4 (cont.).
Group B7.
Microscopic
evaluation
of
oocytes (immature and mature)
P. Coy
15.30h. Practice 4 (cont.).
Groups B3 and B4.
Microscopic
evaluation
of
immature and mature oocytes.
C. Soriano and R. Romar
WEDNESDAY 16 Dec
12.30h. Practice 6 (cont.).
Groups B2 and B3 (12.30h).
Microscopic evaluation of zygotes.
R. Romar and S. Cánovas
15.30h. Practice 6 (cont.).
Groups B1.
Microscopic evaluation of zygotes.
C. Matas
12.30h. Practice 7 (cont.).
Group B2.
ICSI of pig oocytes
FA. García
15.30h. Practice 7 (cont.).
Group B3.
ICSI of pig oocytes
FA. García
12.30h.
Lesson
(Veterinary Farm).
Embryo culture (EC
generalities.
Embryo culture (EC
problems derived from
vitro culture.
D. Rizos (INIA Madrid)
6
I):
II):
in
SUBJECT “IN VITRO MATURATION, IN VITRO FERTILIZATION AND EMBRYO CULTURE”. 9th-22nd December 2015.
Master Course “Biology and Technology of Reproduction in Mammals”. 2015-16.
Theoretical Sessions: Room -1.3. Library (Veterinary Faculty). Practical Sessions: Department of Veterinary Physiology (1st floor, Faculty of Veterinary).
MONDAY 21 Dec
TUESDAY 22 Dec
9.30. Lesson 7.
Embryo culture and development
in humans.
V. Hurtado de Mendoza,
ASEBIR.
9.00h. Practice 8
Group C1 and C2
Morphological evaluation of
embryo development in pig and
cow. R. Romar and C. Soriano
11.30h. Practice 7 (cont).
Group B5.
ICSI of pig oocytes
FA. García
10.15h. Practice 8 (cont.).
Groups C3 and C4.
Morphological evaluation of
embryo development in pig and
cow. R. Romar and S. Cánovas
13.00h. Practice 7 (cont.).
Group B6.
ICSI of pig oocytes
FA. García
WEDNESDAY 23 Dec
THURSDAY 24 Dec
FRIDAY 25 Dec
HOLIDAY
HOLIDAY
HOLIDAY
HOLIDAY
11.30h Mock exam (Physiology
lab, all groups)
12.30h
Manipulation
and
potential
applications
of
oocytes enclosed in preantral
ovarian follicles.
Dr. Luis Vieira (Universidade
Estadual do Ceará, Brasil)
16.00h. Practice 7 (cont.).
Group B7.
FA. García
Master BIOLOGÍA Y TECNOLOGIA DE LA REPRODUCCIÓN EN MAMÍFEROS
Asignatura: Reproducción Asistida en la Especie Humana.
Coordinadora: Marta Mollá (Marta.Molla@ivi.es)
Curso 2015-2016
Del 1-12 febrero 2016
Las clases tendrán lugar en la Clínica IVI-Murcia (biblioteca)
C/ Navegante Macías del Poyo, 5,
Edificio Delfín, 30007 Murcia
Lunes 1 Febrero:
14:50 Apertura del curso: Marta Mollá
15:00-17:00 Estudio de la pareja estéril.
Dra. María Nicolás. Ginecóloga. Unidad de Medicina Reproductiva IVI Murcia
17:00-18:00: Factores endocrinos de la infertilidad.
Dra. Marta Arráez Monllor (Servicio de Endocrinología y nutrición H.U. Virgen de La
Arrixaca)
Martes 2 Febrero:
11:00-12:30: Protocolos de estimulación ovárica controlada.
Dra. Martina Trabalón. Ginecóloga. Unidad de Medicina Reproductiva IVI Murcia
12:30-14:00 Fallo de implantación. Causas y tratamientos.
Dra. Laura Fernández Olmedilla. Ginecóloga. Unidad de Medicina Reproductiva IVI
Murcia.
Miércoles 3 Febrero:
17:00-18:30 Preservación de la fertilidad: Gonadotoxicidad de los tratamientos
oncológicos:
Dr. Jose Luis Alonso. Jefe de servicio de Oncología del Hospital Virgen de la Arrixaca
18:30-20:00 Estimulación Ovárica en pacientes con Cáncer hormono dependiente
Dr Jose L. Landeras Gutiérrez. Director Médico IVI Murcia
Jueves 4 Febrero:
15:00-16:00 Factor masculino
Dr. Juan Carlos Martínez Soto. Director Lab de Andrología IVI Murcia.
16:00-17:00 Inseminación artificial
Dra. Elena Sellés Soriano. Lab de Andrología IVI Alicante.
17:00-18:00 Técnicas de selección espermática
Dr. Juan Carlos Martínez Soto. Director Lab de Andrología IVI Murcia.
Viernes 5 Febrero:
16:00-17:00: Test SCD
Dra. Lourdes Muriel Ríos. Directora Laboratorio de Fecundación in vitro KINGS
COLLEGE Hospital (Londres, U.K.)
17:00 18:00 Salidas profesionales en la UE.
Dra. Lourdes Muriel Ríos. Directora Laboratorio de Fecundación in vitro KINGS
COLLEGE Hospital (Londres, U.K.)
Lunes 8 Febrero
10:00-12:00. Laboratorio de Fecundación in Vitro.
Dra. Beatriz Amorocho Llanos. Supervisora Laboratorio FIV, IVI Murcia.
12:00-13:00. Vitrificación ovocitaria y embrionaria. Usos y aplicaciones clínicas
Inmaculada Campos Ramírez. Directora laboratorio FIV, IVI Almería
Martes 9 Febrero:
16:00-17:00. Criterios morfológicos y no morfológicos de selección de embriones.
Dr. David Gumbao Baño. Embriólogo Clínico, Laboratorio FIV, IVI Murcia
17:00-18:00. Nuevos Sistemas de selección embrionaria.
Julián Marcos. Embriólogo Clínico, Laboratorio FIV, IVI Murcia.
18:00-19:00 Diagnóstico Genético Preimplantacional
Inmaculada Pérez Cano. Directora Laboratorio FIV, IVI Alicante.
Miércoles 10 Febrero:
16:00-17:00 Aspectos Psicológicos de la reproducción asistida.
Marisol Rodenas. Psicóloga, IVI Murcia
17:00-18:00 Nuevas aplicaciones de las técnicas de Fecundación in Vitro
Activación ovocitaria mediante ionóforo de calcio, Cultivo embrionario in vivo.
Biopsia del corpúsculo Polar
Marta Mollá Silva. Directora Laboratorio FIV, IVI Murcia
18:00-19:00 Control de calidad en laboratorio de reproducción.
Dra. Beatriz Amorocho Llanos. Supervisora Laboratorio FIV, IVI Murcia.
Viernes 12 Febrero:
11:00-12:30. Test de compatibilidad genética
Dr. Julio Martín Rodríguez. Head of PGD Genomics. IGENOMIX
12:30-14:00. Test Endometriun Receptivity Assay (ERA)
María Ruíz Alonso. Lab Manager. IGENOMIX
15:30-17:00 Técnicas de diagnóstico molecular en enfermedades genéticas
asociadas a infertilidad humana.
Dra. Inmaculada Campos Galindo. IVIOMICS.
17:00-18:30 Asesoramiento genético/reproductivo, aplicaciones en la práctica
clínica diaria. Indicaciones para el diagnóstico genético prenatal .Nuevos métodos
de análisis genético.
Mª Carmen Martínez Romero. Unidad de Biología Molecular HUVA.
ASPECTOS ÉTICO-LEGALES DEL COMIENZO DE LA VIDA Y DE LA REPRODUCCIÓN ASISTIDA [15/16]:
Estimados alumnos:
La asignatura de Aspectos ético-legales... se imparte en la semana del 15 al 19 de febrero 2016.
Horario: 9,30-13horas.
Lugar: Facultad de Medicina, 3ª planta, Seminario 3.1. Está en la zona central de la planta.
Un cordial saludo
SUBJECT “REPRODUCTIVE BIOTECHNOLOGIES AND RECOVERY OF ENDANGERED SPECIES”. February 22-26th.
Master Course “Biology and Technology of Reproduction in Mammals”. 2015‐16. Monday 22 Feb
Presentation
10.0013.30h
Lesson 1.
Current state of the
reproductive technologies
Dr. Joaquín Gadea
Dpto. Fisiología, UMU
Lesson 2.
Superovulation in equids and
aplications
Dr. Ignacio Giménez Nebot,
Rara Avis Biotecnología
Tuesday 23 Feb
Lesson 4.
Wednesday 24 Feb
Thursday 25 Feb
Lesson 6.
Porcine Artificial Insemination
Reproductive biotechnologies in Mundomar-Benidorm visit.
centers
porcine: sex sorted semen and
D. Pedro José Llamas
Handling of aquatic and wild
embryo transfer
Import
p –Vet
animals
animals.
Dr. Francisco García-Vázquez
Dpto. Fisiología, UMU
Reproductive
methodologies
Lesson 7.
used in zoos
Artiificial insemination in porcine
Lesson 5.
D Pedro José Llamas
D.
D Carlos Barros
D.
Import –Vet
Oestrus siychronization,
artificial insemination and
embryo transfer in bovine
Clinic case
Dra Susana Astiz,
Dra.
Astiz INIA
Low reproductive and
productive efficiency at the
farm: analysis of causes
Practice 2.
Dª.. Sara Crespo, JISAP
D
14.0016.00h
16.0019.00h
L
Lesson
3
3.
Artificial insemination and
embryo transfer in equids
D. Josep Giner Torres
P ti 1.
Practice
1
P ti 3.
Practice
3
Artificial insemination in bovine
Artificial insemination in porcine
Dra. Susana Astiz, INIA
Dr. Francisco García-Vázquez,
Dpto. Fisiología, UMU
Dr. Salvador Ruiz,
Dr
Ruiz
Dpto. Fisiología, UMU
UMU farm
(Boots and boilersuit)
Dª. Sara Crespo, JISAP
(Boots and boilersuit)
Friday 26Feb
Lesson 8.
Contraception in wild animals
Dra. Pilar Coy
Dpto. Fisiología, UMU
Lesson 9.
Interspecies Nuclear Transfer:
uses and limitations for
recovering endangered species
Dr. Sebastian Cánovas, UMU
Lesson 10.
10 12.45h
12 45h
Physiology of reproduction in
dolphins
and
applied
biotechnology
Dra. María Jesús Sánchez
Calabuig, INIA
CRYOPRESERVATION of GAMETES and EMBRYOS (February 29th - March 4th, 2016)
MONDAY
February 29th
TUESDAY
March 1th
WEDNESDAY
March 2th
THURSDAY
March 3th
FRIDAY
March 4th
Practice II
Practice IV
Frozen/Thawed
Semen Evaluation
Theory & Practice (II)
(9.15-11.30 h)
Introduction
Practice Groups Distribution
Frozen/Thawed
Semen Evaluation
Practice VI
(9.30-11.30 h)
Group A: 9.15-11.30 h
Group B: 9.15-11.30 h
Obtaining Oocytes
for Vitrification
Freezing of
Human Semen
Basics of Cryobiology
Sperm Cryopreservation
(Complete Group)
(Complete Group)
J. Gadea
Group C: 12-14 h
Salvador Ruiz (UMU)
Physiology Lab
Cristina Soriano (UMU)
Juan Carlos Martínez
(IVI-Murcia)
Oxidative Stress
and Cryopreservation
Theory II
(12.30-14.30 h)
Joaquín Gadea (UMU)
Freezing of
Horse Semen
Frozen Semen
and Fertility
Jordi Miró
(Univ. Autónoma Barcelona)
Cryopreservation of Gametes
and Embryos in Lab Animals.
Basics and Applications
J. Gadea
Master Classroom
Veterinary Faculty
Gonzalo Moreno Del Val
(Instituto Neurociencias Alicante)
Cryopreservation of
Pluripotent and
Induced Embryonic
Stem Cells
Master Classroom
Veterinary Faculty
Sebastián Cánovas
(UMU)
Theory I
(9.30-14.30 h)
Master Classroom
Veterinary Faculty
J. Gadea
Physiology Lab
Physiology Lab
Physiology Lab
Theory III
(12-14 h)
Theory IV
(12-13.30 h)
Master Classroom
Veterinary Faculty
Practice I
Freezing of
Boar Semen
Group A: 16-18 h
Practice III
(16.30-18.30 h)
Freezing of
Horse Semen
Practice V
Freezing of
Boar Semen
Group B: 15.30-17.30 h
(Complete Group)
Vitrification of
Oocytes and Embryos
(Complete Group)
Group C: 17.30-19.30 h
S. Ruiz
Physiology Lab
Theory & Practice (I)
(16-20 h)
J. Miró
Rodrigo Diego (UMU)
S. Ruiz
Physiology Lab
Physiology Lab
Beatriz Amorocho
David Gumbao
(IVI-Murcia)
Physiology Lab
18 Students (6 Students/Practice Group): Group A (6). Group B (6). Group C (6).
Encuesta
(13.30-14 h)
Evaluación sobre
Satisfacción de la
Actividad Docente
Unidad Calidad
(UMU)
Master Classroom
Veterinary Faculty
ASIGNATURA “Transgénesis, terapia génica, clonación y células madre”.
Del 7 al 11 de Marzo de 2016.
Master “Biología y tecnología de la reproducción en mamíferos”. Curso 2015-16.
Sesiones teóricas: Clase -1.3, Biblioteca. Facultad Veterinaria.
LUNES 7 marzo
9:15 Presentación del curso y
guía docente
MARTES 8 marzo
MIERCOLES 9 marzo
9:15
9:15
Producción
de
aves
Interés de los animales transgénicas
transgénicos en la agricultura y
la medicina.
Modelos de estudio de
enfermedades.
Metodología de la transgénesis:
Microinyección del DNA en Producción
de
conejos
pronúcleo.
transgénicos
Aspectos éticos y morales de la Producción
transgénesis animal
transgénicos
de
JUEVES 10 marzo
9:15
Cerdos transgénicos para xeno- Modelos Animales en
trasplantes
Experimentación. La transgénesis y
clonación en animales de
Dr. Guillermo Ramis. UM
laboratorio.
Uso
de
endonucleasas Células troncales en la
específicas de sitio para la Transgénesis
modificación génica en animales
Células Troncales en vertebrados y
peces CRISPR y su aplicación en sus aplicaciones
animales de granja
Dra. Mª Teresa Paramio. UAB. Dra. Mª Teresa Paramio. UAB.
Barcelona
Barcelona
Dr. Pablo Bermejo-Álvarez. INIA Dr. Miguel Ángel Ramírez, INIA.
Madrid.
Madrid.
15.30
15.30
15.30
Producción de rumiantes
transgénicos
Producción
transgénicos.
aplicaciones
Dra. Mª Teresa Paramio. UAB.
Barcelona
VIERNES 11 marzo
9:15
Clonación: transferencia nuclear
en bovino.
Células madre: ¿qué son?, tipos,
¿Cómo se obtienen y cómo se
cultivan? Aplicaciones.
Reprogramación celular y
transdiferenciación
Dr. Sebastián Cánovas. UM.
14:00. Evaluación docente.
Unidad de Calidad. UM.
15.30
de
cerdos Utilización
de
los Practicas: evaluación microscópica
Metodología y espermatozoides como vectores
de la transgénesis: SMGT.
Dr. Miguel Ángel Ramírez, INIA.
Madrid.
Dr. Joaquín Gadea. UM
Dr. Francisco García-Vázquez.
UM
Evaluación: Examen preguntas cortas sobre los conceptos básicos de la asignatura.
Los alumnos en parejas (2 personas) elaborarán un documento de revisión sobre un tema relacionado con la transgénesis animal, clonación, terapia génica o
células madre. Este documento tendrá un formato de revista científica que si tiene la calidad suficiente y así se desea podrá ser enviada para su evaluación.
Fecha de entrega documento: lunes 20 de mayo. Presentación: 10 min día del examen
Master Biology and Technology of Reproduction (Murcia University. Veterinary Faculty).
Applications of Ultrasonography in Reproductive Biology (April 4-8th, 2016)
MONDAY
April 4th
TUESDAY
April 5th
WEDNESDAY
April 6th
THURSDAY
April 7th
FRIDAY
April 8th
Theory (I) (10 - 13.30 h)
Theory (II) (10 - 13.30 h)
Theory (III) (11.30 - 14 h)
Theory (IV) (10 - 13.30h)
Practice (V) (10 - 13.30 h)
Introduction
Dr. Salvador Ruiz
(UMU)
Ultrasound reproductive in cows
Ultrasound reproductive
in small ruminants
JC Gardón
Ultrasound reproductive
in mares
Dr. Jordi Miró
(UAB)
Revision ecographic images
S Ruiz
Ultrasound in pigs
Dr. Iván Hernández
(Boehringer-Ingelheim)
Basics of ultrasounds
Ultrasound reproductive in pigs
Dr. Juan Carlos Gardón
(Univ. Católica Valencia)
Master Classroom
Veterinary Faculty
OPU in cows
S Ruiz
Master Classroom
Veterinary Faculty
Master Classroom
Veterinary Faculty
Master Classroom
Veterinary Faculty
Practice (I) (16 - 18.30 h)
Practice (II) (16 - 18.30 h)
Practice (III) (15 - 18.30 h)
Practice (IV) (16 - 18.30 h)
Equipment. Probes.
Investigation of female
reproductive tracts and testicles
by ultrasonography in vitro
(samples from slaughterhouse)
JC Gardón, S Ruiz
Rodrigo Diego (UMU)
Physiology Lab
Veterinary Faculty
Ultrasound in cows and goats
JC Gardón, S Ruiz
Ultrasound in mares
J. Miró, R. Diego, S Ruiz,
Angel Cascales
(Mare Farm “La Luna”)
OPU in cows
S Ruiz
Veterinary Farm
Murcia University
Mare Farm “La Luna”
Archena (Murcia)
Veterinary Farm
Murcia University
Pause: Lunch
Scanners: Falco-Vet Esaote. Imago ECM. Easi-Scan Curve BCF Technology.
Nº students: 8.
Note: Practices in farm required that students are equipped with overall and boots.
Veterinary Farm
Murcia University
Dear students,
Welcome to the Epidemiology of Reproductive Health Course of the MSc in Biology &
Technology of Reproduction in Mammals of the UMU. The Course will take place next
week and there will be 5 sessions between Monday, April 11th and Friday, April 15th,
once a day. The classes (Monday, Wednesday, Thursday and Friday) will be held at the
School of Medicine of the UMU (Aula 1.1) between 4pm-8pm. Tuesday session
(epidemiology of animal health) will take place at the regular location, School of
Veterinary (Aula -1.3). The syllabus and the course materials will be uploaded in
"Recursos" in the next days.
Please, do not hesitate to contact us if you have any queries or need any clarification.
II Training School on Epigenetics in
Reproductive Biology
Speakers:
Dept of Reproduction, Obstetrics and
Herd Health, Univ of Ghent, Belgium
Epigenetics Programme, The Babraham
Institute, Cambridge, UK.
Susana Chuva Sousa Lopes Dept of Anatomy and
Embryology, Leiden University Medical Center,
Netherlands.
INIA-Madrid, Spain
Murcia, Spain
Training School on
Epigenetics in
Reproductive Biology
Dept of Animal Reproduction
Dept of Physiology, University of
Epigenetics Programme, The Babraham
Institute, Cambridge, UK.
Simon Andrews , Bioinformatics Unit,The Babraham
Institute, Cambridge, UK
Organizers:
18-21th April, 2016
II Training School on Epigenetics in
Reproductive Biology,
University of Murcia, Spain
18-21th April, 2016, Murcia, Spain
DEGs 789
536
Contact
253
UP
178
María Jiménez-Movilla: mariajm@um.es
Pilar Coy Fuster: pcoy@um.es
Sebastián Cánovas Bernabé: scber@um.es
284
252 (Up)
337
(85 Down)
168
106
DOWN
Pre-registration:
8st to February 28th 2016
II Course on Epigenetics in Reproductive Biology,
Epigenetics in Reproductive Biology,
18-21 April, 2016, Murcia, Spain
18-21 May, 2016, Murcia, Spain
Programme
up: beads purification.
Step IV. Reaction clean
Epigenetic control in embryonic cell
lines. Dr. Susana Chuva Sousa Lopes
Epigenetic re-programming through
the germ cell development and gametogenesis.
Dr. Susana Chuva Sousa Lopes
Impact of DNA methylation in human
testicular tumors of germinal cells.
Registration
The pre-registration period will be opened from
February 8st to February 28th 2016. Applicants
should send an email to mariajm@um.es
including their CV. The accepted applicants will
be able to register from March 7th 2016. The
COST ACTION EPICONCEPT (FA 1201) will provide
500€ grants to selected applicants according to
their CVs. The course will be free for the
participants, including attendance to lectures,
practical exercises and lab consumables. The
official language of the course will be English.
The maximum number of participants will be 15.
Venue
Step V. Biotin capture,
second strand synthesis and PCR amplification.
Step
purification
VI.
Library
Impact of epigenetics in embryonic
development and adaptation.
The theory of bisulphite methylation
data processing and analysis. Dr. Simon Andrews
methylation
analysis
Lab session: Differential
from bisulphite data.
The course will be held on Campus de Espinardo,
University of Murcia, which is just a 15 minute
tramride from the city center.
Theoretical and practical sessions will use
different facilities from Veterinary Schools as well
as from the Central Services for Research Support
located on Campus