Identification Extraction Quantification Post Translational

Multi-Omics approach for the analysis of Serum
Biomolecules in Cardiovascular Diseases
Introduction
Jesús Lavado García, Celia Castañs García, Aleksandra Binek,
Inmaculada Jorge Cerrudo, Alessia Ferrarini, Jesús Vázquez
Cardiovascular Proteomics, CNIC. Melchor Fernández Almagro 3, 28029 Madrid, Spain
Cardiovascular disease is the first-leading cause of death worldwide. Thus research on myocardial infarction represents an
essential key point for the development of novel future therapies promoting the improvement of daily life. The use of the organism
[1]
Sus scrofa (common pig) has been proven highly useful in the study of heart conditions . Here a combined method of proteomics
and metabolomics is applied to the analysis of pig’s serum in order to unveil molecules (proteins and metabolites) involved in
ischemia-reperfusion affection.
Extraction
“If I can stop one heart from breaking,
I shall not live in vain.”
Emily Dickinson
Fractionation
Identification
Post Translational Modification Analysis
Quantification
Conclusion
This technology allows a fast, deep, reproducible
and robust analysis of serum proteome and
metabolome providing new insights to the study of
heart infarction in pig model. It contributes to the
discovery of novel molecules for diagnosis,
prognosis and future therapies. This avant-garde
approach grants applicability to different biological
samples.
Acknowledgements
Thanks to CNIC Institute and its funding with ‘Beca
Máster CNIC’.
References
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749.
[4] García-Marqués F. et al. (2016). Mol Cell Proteomics Molecular &
Cellular Proteomics 15:1740–1760.
[4]