Análisis in silico de lipocalinas de perro, gato, caballo, vaca

Re vista
ARTÍCULO ORIGINAL
Rev Alerg Méx LULTHY"!
Mé xic o
Análisis in silico de lipocalinas de
WLYYVNH[VJHIHSSV]HJHOmTZ[LY`
NHSSPUH7VZPISLLMLJ[VLULSLZ[\KPV
KLSHZLUMLYTLKHKLZHStYNPJHZ
Andrés Sánchez1-3, Ricardo Cardona3, Jorge Sánchez4
9LZ\TLU
(5;,*,+,5;,:! SHZSPWVJHSPUHZWHYLJLUL_WSPJHYSHYLHJ[P]PKHKJY\aHKHLU[YLHUPTHSLZJVTVNH[V`WLYYVWLYVWVJVZLOHLZ[\KPHKV
HJLYJHKLZ\WHWLSLUSHYLHJ[P]PKHKJY\aHKHJVUV[YVZHUPTHSLZ`
Z\LMLJ[VJSxUPJV
6)1,;0=6:! HUHSPaHYWVY[tJUPJHZIPVPUMVYTm[PJHZSHPKLU[PKHKLU[YL
SPWVJHSPUHZKLKPMLYLU[LZLZWLJPLZ`L_WSVYHYSH\[PSPKHKKLLZ[HZ[tJUPJHZ
LULSLZ[\KPVKLSHZHSLYNPHZ
4(;,90(3@4i;6+6! LZ[\KPVin silicoLULSX\LZLI\ZJHYVU las seJ\LUJPHZKLSPWVJHSPUHZ\[PSPaHUKVLSWYVNYHTH)3(:;3HZZLJ\LUJPHZ
KLWYV[LxUHZZLHSPULHYVUJVULSWYVNYHTH*3<:;(36TLNH]LYZP}U
KL<UP7YV[3HZZLJ\LUJPHZIHZLKLSVZHSPULHTPLU[VZM\LYVUSHZ
SPWVJHSPUHZKLWLYYVZ`NH[VZ3HPKLU[PKHKLU[YLSHZSPWVJHSPUHZZLJVTWHY}JVUSHMYLJ\LUJPHKLZLUZPIPSPaHJP}UHHUPTHSLZLU\UHWVISHJP}U
KLWHJPLU[LZHStYNPJVZ
9,:<3;(+6:!SHZZLJ\LUJPHZTVZ[YHYVUPKLU[PKHKLZLU[YL`
3VZ]HSVYLZTmZHS[VZZLLUJVU[YHYVULU[YL*HUM-LSK`-LSK
,X\J3HPKLU[PKHKTmZIHQHM\LJVUSHZSPWVJHSPUHZW\YW\YPUH
`WYV[LxUHKL\UP}UHSYL[PUVSKLSNHSSVTLUVYKL6IZLY]HTVZ
\UHYLSHJP}ULU[YLLSWH[Y}UKLZLUZPIPSPaHJP}U`LSNYHKVKLPKLU[PKHK
LU[YLSHZLZWLJPLZLZ[\KPHKHZ
*65*3<:065,:!HWHY[PYKLSVZLZ[\KPVZIPVPUMVYTm[PJVZ`SVZWH[YVULZ
KLZLUZPIPSPaHJP}ULUJVU[YHKVZZLWYVWVULX\L-LSK`,X\JZVU
WVZPISLZ HSLYNLUVZ TH`VYLZ WHYH NH[V ` JHIHSSV LU SH WVISHJP}U KLS
[Y}WPJV`JVTWHY[LUHS[HYLHJ[P]PKHKJY\aHKHJVU*HUM+LIPKVHX\L
LZ[VZYLZ\S[HKVZWYV]PLULUKLTVKLSVZWYLKPJ[P]VZKLILUJVUÄYTHYZL
JVULZ[\KPVZin vitro e in vivo.
7(3()9(:*3(=,!HSLYNPHH]LZIPVPUMVYTm[PJHJHIHSSVNH[VNHSSPUH
PKLU[PKHKSPWVJHSPUHZTHZJV[HZZLUZPIPSPaHJP}U
www.nietoeditores.com.mx
1
Universidad deů Magdalena, Departamento de
Medicina, Santa Marta, Colombia.
2
Corporación Universitaria Rafael Núñez, Programa
de Medicina, Cartagena, Colombia.
3
Grupo de Alergología Clínica y Experimental. IPS
hŶŝǀĞƌƐŝƚĂƌŝĂ͕ hŶŝǀĞƌƐŝĚĂĚ ĚĞ ŶƟŽƋƵŝĂ͕ DĞĚĞůůşŶ͕
Colombia.
4
Grupo de Alergología Experimental e InmunogeŶĠƟĐĂ͕ /ŶƐƟƚƵƚŽ ĚĞ /ŶǀĞƐƟŐĂĐŝŽŶĞƐ /ŶŵƵŶŽůſŐŝĐĂƐ͕
Universidad de Cartagena, Cartagena, Colombia.
Recibido: 25 de mayo 2015
Aceptado: 27 de noviembre 2015
Correspondencia
Dr. Jorge Sánchez
jotamsc@yahoo.com
ƐƚĞĂƌơĐƵůŽĚĞďĞĐŝƚĂƌƐĞĐŽŵŽ
Sánchez A, Cardona R, Sánchez J. Análisis in silico de lipocalinas de perro, gato, caballo, vaca,
hámster y gallina. Posible efecto en el estudio de
las enfermedades alérgicas. Rev Alerg Méx. 2016
ene-mar;63(1):1-10.
1
2016 enero-marzo;63(1)
Revista Alergia México
9L](SLYN4t_1HU4HY"!
In silicoHUHS`ZPZVM[OLPKLU[P[`VM
SPWVJHSPUVMKVNJH[OVYZLJV^OHTZ[LY
HUKOLU7VZZPISLYVSLPUHSSLYNPJKPZLHZLZ
Andrés Sánchez1-3, Ricardo Cardona3, Jorge Sánchez4
Abstract
)(*2.96<5+! 3PWVJHSPUZ ZLLT [V L_WSHPU [OL JYVZZYLHJ[P]P[`
IL[^LLU ZVTL WL[Z Z\JO HZ JH[ HUK KVN /V^L]LY P[Z YVSL PU V[OLY
HUPTHSZHUKP[ZWVZZPISLJSPUPJHSPTWHJ[PUHSSLYN`KPZLHZLZOH]LILLU
ZJHYJLS`Z[\KPLK
6)1,*;0=,! ;V HUHS`aL I` IPVPUMVYTH[PJZ [LJOUPX\LZ [OL PKLU[P[`
IL[^LLUSPWVJHSPUVMZVTLHUPTHSZHUK[VL_WSVYL[OLJSPUPJHSPTWHJ[
VUHSSLYNPJKPZLHZLZ
4(;,90(3(5+4,;/6+! (Uin silicoZ[\K`^HZKVUL[VZLHYJOMVY
SPWVJHSPUZLX\LUJLZ\ZPUN[OL)3(:;WYVNYHTVM5*)0+H[HIHZL;OL
WYV[LPU ZLX\LUJLZ ^LYL HSPNULK ^P[O *3<:;(3 6TLNH <UP7YV[ ]LYZPVUZVM[^HYL;OLIHZLZLX\LUJLZMVYHSPNUTLU[Z^LYLSPWVJHSPUZ
KVNZHUKJH[Z;OLKLÄULKWLYJLU[HNLPKLU[P[`^HZJVTWHYLK^P[O[OL
MYLX\LUJ`VMZLUZP[PaH[PVU[VHUPTHSZL_WVZLKPUHWVW\SH[PVUVM
WH[PLU[Z^P[OZ\ZWLJ[LKHSSLYNPJKPZLHZLZ
9,:<3;:! 0KLU[P[PLZIL[^LLUZLX\LUJLZ^LYL[V;OLOPNOLZ[]HS\LZ^LYLMV\UK^P[O*HUM-LSKHUK-LSK,X\J
;OLSV^LYPKLU[P[`^HZMV\UK^P[OSPWVJHSPUWVYW\YPUHUKYL[PUVS
IPUKPUN#>LVIZLY]LKHYLSH[PVUZOPWIL[^LLUZLUZP[PaH[PVUHUK
[OLWLYJLU[PKLU[P[`IL[^LLU[OLZWLJPLZZ[\KPLK
*65*3<:065:! 3PWVJHSPUZ HZ *HU M -LS KL HUK ,X\ J ZLLT
[VWSH`HUPTWVY[HU[YVSLPU[OLJYVZZYLHJ[P]P[`[VJH[OVYZLHUKKVN
I\[UV[MVY[OLJVZLUZP[PaH[PVU[VOHTZ[LYJV^VYIPYKZ-LSKLHUK
,X\JJV\SKILHWYL]HSLU[HSSLYNLUMVYOVYZLHUKJH[;OLZLYLZ\S[Z
JVTLMYVTWYLKPJ[P]LHUHS`ZPZHUKT\Z[ILJVUÄYTLKI`in vitroHUK
in vivoZ[\KPLZ
2,@>69+:! HSSLYN`" IPYKZ" IPVPUMVYTH[PJZ" OVYZL" JH[" OLU" PKLU[P[`"
lipocalin; pets; sensitization
ANTECEDENTES
3H MYLJ\LUJPH KL H[VWPH ` LUMLYTLKHKLZ HStYNPJHZJVTVHZTHYPUP[PZ`JVUQ\U[P]P[PZLZHS[H
` WHYLJL X\L SH PUJPKLUJPH LZ[m H\TLU[HUKV
LZWLJPHSTLU[L LU SVZ WHxZLZ PUK\Z[YPHSPaHKVZ1
2
1
Universidad de Magdalena, Departamento
de Medicina, Santa Marta, Colombia.
2
Corporación Universitaria Rafael Núñez,
Programa de Medicina, Cartagena, Colombia.
3
Grupo de Alergología Clínica y Experimental. IPS Universitaria, Universidad de
ŶƟŽƋƵŝĂ͕DĞĚĞůůşŶ͕ŽůŽŵďŝĂ͘
4
Grupo de Alergología Experimental e InŵƵŶŽŐĞŶĠƟĐĂ͕/ŶƐƟƚƵƚŽĚĞ/ŶǀĞƐƟŐĂĐŝŽŶĞƐ
Inmunológicas, Universidad de Cartagena,
Cartagena, Colombia.
Correspondence
Dr. Jorge Sánchez
jotamsc@yahoo.com
,U[YLSHZWYPUJPWHSLZM\LU[LZKLHSLYNLUVZLZ[mU
SHZ THZJV[HZ" L_PZ[L \U U‚TLYV JYLJPLU[L KL
MHTPSPHZLU3H[PUVHTtYPJHJVUTHZJV[HZLUZ\Z
JHZHZ:PSHL_WVZPJP}UWYVSVUNHKHHTHZJV[HZLZ
\UMHJ[VYKLYPLZNVKLVWYV[LJ[VYJVU[YHSHHWHYPJP}UKLHSLYNPHZLZ\U[LTHH‚ULUKPZJ\ZP}U"
Re vista
Sánchez A y col. Análisis in silico de lipocalinas en alergia a mascotas
ZPULTIHYNVLU[YLSVZWHJPLU[LZJVUZVZWLJOHKL
HSLYNPHZSHZN\xHZTtKPJHZYLJVTPLUKHUYLHSPaHY
SHZ TLKPKHZ KL L]P[HJP}U2 WLYV WVY KPMLYLU[LZ
TV[P]VZLU[YLLSSVZLTVJPVUHSLZSHTH`VYxHKL
SVZWHJPLU[LZ[PLULKPÄJ\S[HKLZWHYHJVUZLN\PY
LZ[LVIQL[P]V"HKLTmZSHLMLJ[P]PKHKJSxUPJHKL
LZ[HYLJVTLUKHJP}UUVLZJSHYH3
Mé xic o
IPVPUMVYTm[PJHZSHPKLU[PKHKLU[YLSHZSPWVJHSPUHZ
KLWLYYVNH[VJHIHSSV]HJHOmTZ[LY`NHSSPUH
`Z\WVZPISLWHWLSLUSHZHSLYNPHZHHUPTHSLZLU
SHWVISHJP}UKLS[Y}WPJV
MATERIAL Y MÉTODO
Secuencias de lipocalinas
<UWYVISLTHHKPJPVUHSWHYHSVNYHYSHZTLKPKHZ
KL L]P[HJP}U LZ SH YLHJ[P]PKHK JY\aHKH LU[YL
HSN\UHZTHZJV[HZLZWLJPHSTLU[LLU[YLNH[VZ`
WLYYVZX\LZVUSHZTHZJV[HZX\LJVUTmZMYLcuencia generan alergias en Europa.,ULZ[\KPVZ
WYL]PVZVIZLY]HTVZX\LSHZLUZPIPSPaHJP}UHTHZJV[HZ[HTIPtULZWYL]HSLU[LLUHSN\UHZJP\KHKLZ
KL3H[PUVHTtYPJH3HZSPWVJHSPUHZZVUWYV[LxUHZ
X\L [YHUZWVY[HU WLX\L|HZ TVStJ\SHZ JVTV
LZ[LYVPKLZYL[PUVPKLZ`SxWPKVZ,Z[HMHTPSPHKL
WYV[LxUHZLZ[mWYLZLU[LLUSHTH`VYWHY[LKLSVZ
THTxMLYVZHSPN\HSX\LLUIHJ[LYPHZULTH[VKVZ
L PUZLJ[VZ ` ZVU HSLYNLUVZ PTWVY[HU[LZ JVTV
JH\ZHKLSHYLHJ[P]PKHKJY\aHKHLU[YLJHIHSSV
NH[V`WLYYV:PULTIHYNVWVJVZLZHILHJLYJH
KLSWHWLSKLSHZSPWVJHSPUHZLUSHZLUZPIPSPaHJP}U
HV[YHZM\LU[LZJVT\ULZLU3H[PUVHTtYPJH
3VZ HUmSPZPZ IPVPUMVYTm[PJVZ LU[YL SVZ X\L ZL
PUJS\`LU SVZ HUmSPZPZ in silico ZPT\SHJP}U WVY
JVTW\[HKVYHZZVU\UHOLYYHTPLU[H‚[PSWHYHLS
TVKLSHTPLU[V`SHWYLKPJJP}UKLSHLZ[Y\J[\YHKL
SHZWYV[LxUHZ,Z[HZOLYYHTPLU[HZW\LKLUH`\KHY
HWYLKLJPYHSLYNLUVZTH`VYLZWV[LUJPHSLZ`W\LKLUL_WSPJHYSHYLHJ[P]PKHKJY\aHKHLU[YLKVZV
TmZM\LU[LZKLHSLYNLUVZ:PLU\UHWVISHJP}U
SHMYLJ\LUJPHKLZLUZPIPSPaHJP}UH]HYPHZM\LU[LZ
LZ JVUVJPKH SVZ HUmSPZPZ KL HSPULHTPLU[V ` SH
PKLU[PKHKLU[YLSHZWYV[LxUHZW\LKLUZLYLSWYPTLY
WHZV WHYH PKLU[PÄJHY SVZ HSLYNLUVZ WYPUJPWHSLZ
X\LL_WSPJHUSVZWH[YVULZKLZLUZPIPSPaHJP}U`
JVZLUZPIPSPaHJP}U LU[YL SHZ LZWLJPLZ 7VZ[LYPVYTLU[LLZULJLZHYPVKLTVZ[YHYZPSVZHSLYNLUVZ
PKLU[PÄJHKVZ JVTV WYVIHISLZ ZVU YLHSTLU[L
HSLYNLUVZTH`VYLZTLKPHU[LLZ[\KPVZin vivo. En
LZ[LLZ[\KPVL_WSVYHTVZWVYTLKPVKL[tJUPJHZ
,Z[\KPVin silicoLULSX\LZLI\ZJHYVUSPWVJHSPUHZ\[PSPaHUKVLSWYVNYHTH)3(:;KLSHIHZL
5*)0JVUSHZZLJ\LUJPHZ]HSPKHKHZOHZ[HLS
KLUV]PLTIYLKL:}SVZLPUJS\`LYVUSHZ
WYV[LxUHZ HSLYNtUPJHZ JVU \UH PKLU[PÄJHJP}U
JVTWSL[HKLSHZLJ\LUJPHKL(95TLUZHQLYVKL
WLYYVNH[VJHIHSSVOmTZ[LY`]HJH(KLTmZZL
PUJS\`LYVUKVZSPWVJHSPUHZKLNHSSPUHUVPKLU[PÄJHKHZJVTVHSLYNtUPJHZW\YW\YPUH`WYV[LxUHKL
\UP}UHYL[PUVS!9)7WHYH[LULYZLJ\LUJPHZJVTWHYH[P]HZKLLZ[LNY\WV[H_VU}TPJV3HZLU[YHKHZ
KLZLJ\LUJPHZWHYJPHSLZVJVY[LZHS[LYUH[P]VZZL
L_JS\`LYVU(SN\UHZSPWVJHSPUHZWV[LUJPHSTLU[L
HSLYNtUPJHZJVTV,X\JUVZLPUJS\`LYVULU
LZ[LLZ[\KPVKLIPKVHX\LSHZLJ\LUJPHYLWVY[HKH
LUSHIHZL5*)0LZPUJVTWSL[H
3HZZLJ\LUJPHZWYV[LPJHZZLHSPULHYVU\ZHUKVLS
WYVNYHTH *9<:;(3 6TLNH ]LYZP}U KL
<UP7YV[3HZZLJ\LUJPHZIHZLWHYHSVZHSPULHTPLU[VZM\LYVUSHZSPWVJHSPUHZKLWLYYV`NH[VKLIPKV
HX\LZVUSHZTHZJV[HZTmZJVT\ULZLUSHZJHZHZ
Análisis filogenéticos
3VZHUmSPZPZÄSVNLUt[PJVZIHZHKVZLUSHZZLJ\LUJPHZKL(95ZLJ\LUJPHZKLU\JSL}[PKVZZL
YLHSPaHYVUTLKPHU[LLSTt[VKVKLYLJVUZ[Y\JJP}U
KL 5LPNOIVY1VPUPUN JVU LS WYVNYHTH 4,.(
(molecular evolutionary genetics analysis) ]LYZP}U
\ZHUKVLSTt[VKVbootstrapJVTVWY\LIHKL
ÄSVNLUPHJVUYLWSPJHJPVULZ7HYHKL[LYTPUHY
SHKPZ[HUJPHÄSVNLUt[PJH\[PSPaHTVZLSTt[VKVKL
SH Tm_PTH WYVIHIPSPKHK JVTW\LZ[H [LUPLUKV
LU J\LU[H LS U‚TLYV KL Z\Z[P[\JPVULZ KL IHZL
3
2016 enero-marzo;63(1)
Revista Alergia México
WVYJHKHZP[PV;VKHZSHZWVZPJPVULZJVULZWHJPVZ
]HJxVZ ZL LSPTPUHYVU KLSLJPVULZ JVTWSL[HZ
7HYH [VKVZ SVZ JY\JLZ SHZ WVZPJPVULZ KL JVK}U
PUJS\PKHZWHYHSVZHUmSPZPZM\LYVUWYPTLYVZLN\U
KV[LYJLYVZLJ\LUJPHZUVJVKPÄJHU[LZ+LIPKV
HSU‚TLYVKLZLJ\LUJPHZKLSPWVJHSPUHZ\[PSPaHKHZ
UVYLHSPaHTVZZ\IHUmSPZPZÄSVNLUt[PJVZ
3L[PL0UT\UV[LR4HKYPK,ZWH|H`SHZYLJVTLUKHJPVULZPU[LYUHJPVUHSLZWHYHZ\SLJ[\YHOHI}U
TTTH`VYX\LLSJVU[YVSULNH[P]V ;VKVZ
SVZ WHJPLU[LZ Z\ZWLUKPLYVU SH HKTPUPZ[YHJP}U
KLHU[POPZ[HTxUPJVZ\V[YVZMmYTHJVZLS[PLTWV
YLX\LYPKVHU[LZKLSHWY\LIH
Consideraciones éticas
Selección de la población
<ZHTVZ\UHJVOVY[LKLWHJPLU[LZWYL]PHTLU[L
YLWVY[HKH ` HNYLNHTVZ WHJPLU[LZ U\L]VZ YLJS\[HKVZK\YHU[LLSWLYPVKVKLQ\UPVKLH
THYaVKL9LJVSLJ[HTVZWHJPLU[LZJVU
KPHNU}Z[PJVKLHZTHYPUP[PZVKLYTH[P[PZH[}WPJH
X\LHZPZ[PLYVUHSZLY]PJPVKL(SLYNVSVNxH*SxUPJH
KLSH<UP]LYZPKHKKL(U[PVX\PH4LKLSSxU*VSVTIPH`YLX\PYPLYVU\UHWY\LIHJ\[mULHJVU
HSLYNLUVZWHYHPKLU[PÄJHYSVZWVZPISLZHSLYNLUVZ
HTIPLU[HSLZ+\YHU[LSHJP[HWHYHSHWY\LIHZL
PU[LYYVN}HSVZWHJPLU[LZHJLYJHKLSVZKL[VUHU[LZZVZWLJOVZVZ`SHL_PZ[LUJPHKLTHZJV[HZLU
JHZHVSHL_WVZPJP}UPUKPYLJ[HHHUPTHSLZ3\LNV
KLSHWY\LIHJ\[mULHSVZWHJPLU[LZYLJPIPLYVU
PUMVYTHJP}U KL THULYH ]LYIHS ` WVY LZJYP[V
HJLYJHKLSHZTLKPKHZKLJVU[YVSHTIPLU[HSWHYH
SHZM\LU[LZHSHZX\LLZ[\]PLYVUZLUZPIPSPaHKVZ
*VTV\UJYP[LYPVKLPUJS\ZP}UH[VKVZSVZWHJPLU[LZZLSLZL]HS\}SHZLUZPIPSPaHJP}UHBlomia
tropicalis, Dermatophagoides pteronyssinus,
Dermatophagoides farinae LWP[LSPV KL NH[V
` WLYYV. 0UJS\PTVZ L_[YHJ[VZ HKPJPVUHSLZ KL
animales en los pacientes con contacto o con
ZVZWLJOH KL ZxU[VTHZ WVY V[YVZ HUPTHSLZ WVY
LQLTWSV JHIHSSV OmTZ[LY WmQHYVZ NHSSPUHZ
L[J,SKPHNU}Z[PJVZLLZ[HISLJP}KLHJ\LYKVJVU
SHZN\xHZ.05(KLHZTH^^^NPUHZ[OTHVYN
(90(KLYPUP[PZ6LS*VUZLUZV3H[PUVHTLYPJHUVKL
*VUQ\U[P]P[PZ`SVZJYP[LYPVZKL/HUPÄU`9HQRH
KLKLYTH[P[PZH[}WPJH
7HYHSHWY\LIHJ\[mULHPU[YHLWPKtYTPJH\ZHTVZ
SVZL_[YHJ[VZLZ[HUKHYPaHKVZKLSVZSHIVYH[VYPVZ
4
7HYHSHYL]PZP}UKLSHZOPZ[VYPHZJSxUPJHZ`SVZ
KH[VZKLSVZWHJPLU[LZZLVI[\]VSHHWYVIHJP}U
KLSJVTP[tKLt[PJHKLSHPUZ[P[\JP}UOVZWP[HSHYPH 3HZ ZLJ\LUJPHZ ZL [VTHYVU KL SH IHZL
KLKH[VZKL5*)0WVYSVX\LZVUKLHJJLZV
abierto.
Análisis estadísticos
3VZHUmSPZPZZLYLHSPaHYVUJVULSWYVNYHTH0)4
:7::]LYZP}UWHYH>PUKV^Z3VZYLZ\S[HKVZ
ZLL_WYLZHYVUJVTVWVYJLU[HQLZKLMYLJ\LUJPH
`LUU‚TLYVZHIZVS\[VZ:L\ZHYVUHUmSPZPZKL
JVYYLSHJP}UWY\LIHKLr2VYLNYLZPVULZSVNxZ[PJHZ
T\S[P]HYPHISLZWHYHTLKPYSHZKPMLYLUJPHZLU[YL
los grupos.
RESULTADOS
Identidad de las lipocalinas
3HZ SPWVJHSPUHZ ZLSLJJPVUHKHZ ZL T\LZ[YHU LU
LS *\HKYV :L YLHSPaHYVU HSPULHTPLU[VZ
LU[YL SHZ SPWVJHSPUHZ PUJS\PKHZ 3HZ ZLJ\LUJPHZ
IHZLZ\[PSPaHKHZM\LYVUSHZSPWVJHSPUHZKLWLYYV
` NH[V *\HKYV 3HZ ZLJ\LUJPHZ TVZ[YHYVU
\UHNYHU]HYPHIPSPKHKLU[YLSHZPKLU[PKHKLZ
H3HTH`VYPKLU[PKHKZLLUJVU[Y}LU[YL
SVZHSPULHTPLU[VZKL*HUM-LSKJVU]HSVYLZ
KL`-LSK,X\JJVUZLN\PKVWVY
*HUM-LSKJVU`*HUM,X\JJVU
-PN\YH3HTLUVYPKLU[PKHKZLLUJVU[Y}
LU[YL SVZ HSPULHTPLU[VZ WHYH WVYW\YPUH ` 9)7
JVUSHZV[YHZLZWLJPLZX\LM\LTLUVYKL
*\HKYV`
Re vista
Mé xic o
Sánchez A y col. Análisis in silico de lipocalinas en alergia a mascotas
*\HKYV. 3PWVJHSPUHZZLSLJJPVUHKHZWHYHSVZHSPULHTPLU[VZ
3PWVJHSPUH
Código
)HZLKLKH[VZ
ZLJ\LUJPH(95T
Entrada
(Swissprot)
5‚TLYVKL
HTPUVmJPKVZ
Perro (Canis familiaris)
(-
(-
.< +7)/
/,
/).
.H[V(Felis domesticus o catus)
(33F-,3*(
(@ 8=-/
,+AF-,3*(
.<
,+A
)V]PUL(Bos taurus)
(33F)6=05
3
8
Caballo (Equus caballus)
(33F/69:,
<
8 .HSSPUHNHSSV(Gallus gallus o domesticus)
7<97F*/0*2
4
7 9,;F*/0*2
? 7
/mTZ[LY(Phodopus sungorus)
:A@+F7/6:<
2-
:A@+
*HUM
*HUM
*HUM
*HUM
(33F*(5-(
(33F*(5-(
+7)/F*(5-(
/).F*(5-(
-LSK
-LSK
)VZK
,X\J
7\YW\YPUH
9)7
7OVZR+H
3PWVJHSPUHZZLSLJJPVUHKHZKLKPMLYLU[LZHUPTHSLZWHYHHUmSPZPZIPVPUMVYTm[PJVZ
9)7!WYV[LxUHKL\UP}UHSYL[PUVSretinol binding proteins
*\HKYV. 0KLU[PKHKLU[YLSHZSPWVJHSPUHZKLWLYYV`NH[V
(SPULHTPLU[V
0KLU[PKHK 5‚TKL
5‚TKL
HTPUVmJPKVZ HTPUVmJPKVZ
idénticos
ZPTPSHYLZ
Perro-gato
*HUM-LSK
*HUM-LSK
*HUM-LSK
*HUM-LSK
*HUM-LSK
*HUM-LSK
*HUM-LSK
*HUM-LSK
23
61
23
110
130
61
66
62
*VUSHSPWVJHSPUHKLOmTZ[LY7OVZR+HSH
PKLU[PKHKJVUV[YHZLZWLJPLZ[HTIPtUM\LIHQH!
*HUM7OVZR+H!-LSK7OVZ
R+H!3HSPWVJHSPUHKLSH]HJH)VZKTVZ[Y}]HSVYLZLU[YL`SHTH`VYPKLU[PKHK
ZLLUJVU[Y}JVU*HUMJVU\U]HSVYKL
*\HKYVZ`
Análisis filogenéticos
,SmYIVS}W[PTVJVU\UHSVUNP[\KKLYHTPÄJHJP}U
PN\HSH ZLT\LZ[YHLUSH-PN\YH
,SWVYJLU[HQLKLmYIVSLZYLWSPJHKVZYLWL[PJPVULZZLT\LZ[YHLUSH-PN\YH,SmYIVSZL
T\LZ[YHHLZJHSHSHSVUNP[\KKLSHYHTPÄJHJP}U
YLWYLZLU[HSHKPZ[HUJPHL]VS\[P]HLUSHJHU[PKHK
KLJHTIPVZHJ\T\SHKVZ"ZPULTIHYNVZLKLIL
[LULYLUJ\LU[HX\LLSTt[VKV5LPNOIVY1VPUPUN
Z}SVOHJLHNY\WHJPVULZKL¸TPLTIYVZZPTPSHYLZ¹
ZPU [VTHY LU J\LU[H LS YLSVQ TVSLJ\SHY WVY SV
X\L SH KPZ[HUJPH L]VS\[P]H LZ Z}SV HWYV_PTHKH
KL HJ\LYKV JVU SH PKLU[PKHK KL SHZ WYV[LxUHZ
LZ[\KPHZ/\IVWVZPJPVULZLUSHIHZLÄUHS
Características de la población y
sensibilización a animales
+LSVZWHJPLU[LZ LYHUT\QLYLZ*\HKYVZ
`3HLKHKTLKPHM\LKLH|VZ3HTH`VYxH
KLSVZWHJPLU[LZ[LUxHULUMLYTLKHKLZYLZWPYH[VYPHZ
5
2016 enero-marzo;63(1)
Revista Alergia México
-PN\YH,Z[Y\J[\YHKLHSPULHTPLU[VLU[YL*HUM-LSK`,X\J3VZHTPUVmJPKVZPKLU[PÄJHKVZLZ[mUPUKPJHKVZJVU¸*¹SVZHTPUVmJPKVZZPTPSHYLZLZ[mUPUKPJHKVZJVU¸!¹`YLZHS[HKVZLUNYPZVZJ\YV
`WHJPLU[LZ[LUxHUZxU[VTHZYLZWPYH[VYPVZ`J\[mULVZ"WHJPLU[LZ[LUxHU
H[VWPHWHJPLU[LZM\LYVUWVZP[P]VZH\UV
V]HYPVZHUPTHSLZ,SWLYYVU$`LSNH[V
U$ M\LYVUSHZM\LU[LZJH\ZHU[LZJVUTmZ
MYLJ\LUJPHKLZLUZPIPSPaHJP}ULU[YLTHZJV[HZ"
WHJPLU[LZWVZP[P]VZHNH[VSVM\LYVU[HTIPtUHWLYYV
:LPZWHJPLU[LZM\LYVUWVZP[P]VZHJHIHSSV`[VKVZ
LSSVZSVM\LYVUHNH[V`WLYYV*\H[YVKLLSSVZ[LUxHU
\UHHS[HL_WVZPJP}UHJHIHSSV"WHJPLU[LZ
M\LYVUWVZP[P]VZHWmQHYVZKLLSSVZM\LYVUWVZP[P]VZHNH[VVWLYYV"WHJPLU[LZWVZP[P]VZHWLYYV
YLZ\S[HYVUZLUZPIPSPaHKVZHmJHYVZ`[VKVZSVZ
WHJPLU[LZWVZP[P]VZHNH[VSVM\LYVUHSVZmJHYVZ"
WHJPLU[LZLZ[\]PLYVUL_W\LZ[VZHHSN‚U
HUPTHS!HWLYYVHNH[V
HWmQHYVZ`HV[YVZHUPTHSLZJVTV
JHIHSSVOmTZ[LY`JVULQV5VW\KPTVZL]HS\HYZP
L_PZ[xH\UHYLSHJP}ULU[YLSHL_WVZPJP}UHTHZJV[HZ
`SVZZxU[VTHZKLIPKVHX\LSHWVISHJP}ULZ[HIH
JVUMVYTHKH [V[HSTLU[L WVY WHJPLU[LZ HStYNPJVZ
` UV OHIxH JVU[YVSLZ 3H ‚UPJH HZVJPHJP}U LU[YL
L_WVZPJP}U`ZLUZPIPSPaHJP}UM\LHWmQHYVZW#
TLKPH!0* !
6
Relación entre identidad de lipocalinas y
patrón de sensibilización
6IZLY]HTVZ X\L LS WVYJLU[HQL KL PKLU[PKHK KL
HSN\UHZSPWVJHSPUHZLZ[mLZ[YLJOHTLU[LYLSHJPVUHKV
JVULSWH[Y}UKLZLUZPIPSPaHJP}U3HTH`VYPKLU[PKHKZLLUJVU[Y}WHYH*HUM-LSK`,X\J
JVUMYLJ\LUJPHHS[HKLJVZLUZPIPSPaHJP}UHWLYYV
NH[V`JHIHSSVWTLUVYKL3HZSPWVJHSPUHZ
KLNHSSPUHOmTZ[LY`]HJH[LUxHUWVJHPKLU[PKHK
`WVJHMYLJ\LUJPHKLJVZLUZPIPSPaHJP}UJVUNH[V`
WLYYV+LIPKVHX\LLZ[HHZVJPHJP}UWVKYxHLZ[HY
YLSHJPVUHKHJVULSNYHKVKLL_WVZPJP}UYLHSPaHTVZ \U Z\IHUmSPZPZ JVUZPKLYHUKV SH L_WVZPJP}U
HUPTHSJVTV\UHJV]HYPHISL`UVVIZLY]HTVZ\UH
YLSHJP}U ZPNUPÄJH[P]H LU[YL SH L_WVZPJP}U KPYLJ[H
HJHIHSSVOmTZ[LY]HJHNHSSPUHNH[VVWLYYV`
sensibilización.
DISCUSIÓN
3VZ WH[YVULZ KL ZLUZPIPSPaHJP}U W\LKLU ]HYPHY
KL HJ\LYKV JVU SHZ JVUKPJPVULZ NLVNYmÄJHZ `
ZVJPVJ\S[\YHSLZ KL \UH YLNP}U10-12 +L HJ\LYKV
Re vista
Mé xic o
Sánchez A y col. Análisis in silico de lipocalinas en alergia a mascotas
*\HKYV.0KLU[PKHKLU[YLSPWVJHSPUHZ\ZHUKVSHZZLJ\LUJPHZ
KLSWLYYVJVTVIHZL
(SPULHTPLU[V
*HUM)VZK
*HUM)VZK
*HUM)VZK
*HUM)VZK
*HUM,X\J
*HUM,X\J
*HUM,X\J
*HUM,X\J
*HUM7\YW\YPU
*HUM7<9
*HUM7\YW\YPU
*HUM7<9
*HUM7\YW\YPU
*HUM7<9
*HUM7\YW\YPU
*HUM7<9
*HUM7OVZR+H
*HUM7OVZR+H
*HUM7OVZR+H
*HUM7OVZR+H
0KLU[PKHK 5‚TKL
5‚TKL
HTPUVmJPKVZHTPUVmJPKVZ
idénticos
ZPTPSHYLZ
Caballo-bovino
31
Perro-caballo
26
111
Perro-gallina
13
13
13
36
33
7LYYVOmTZ[LY
31
13
21
63
63
63
66
63
60
JVULS4PUPZ[LYPVKL:HS\KKL*VSVTIPH^^^
TPUWYV[LJJPVUZVJPHSNV]JV`SH:VJPLKHK7YV[LJ[VYHKL(UPTHSLZHSYLKLKVYKL\UHKLJHKH
[YLZJHZHZLULS=HSSLKL(I\YYm[PLULTHZJV[HZ
WLYYVZ`NH[VZ`[PLULHSTLUVZKVZTHZJV[HZKLLZWLJPLZKPMLYLU[LZ,Z[VZ
KH[VZZ\NPLYLUX\LOH`\UHHS[HJVUJLU[YHJP}U
KLWHY[xJ\SHZKLYP]HKHZKLHUPTHSLZLULSHPYL
KL4LKLSSxU:PULTIHYNVUVLUJVU[YHTVZ\UH
YLSHJP}UKPYLJ[HLU[YLSHMYLJ\LUJPHKLL_WVZPJP}U
H NH[VZ V WLYYVZ ` ZLUZPIPSPaHJP}U ,Z[V W\LKL
L_WSPJHYZLWVYSHHS[HMYLJ\LUJPHKLL_WVZPJP}U
PUKPYLJ[HLUSHLZJ\LSHLS[YHIHQVVLS]LJPUKHYPV
X\LLZKPMxJPSKLJ\HU[PÄJHY`JVUZPKLYHUKVSVZ
KH[VZ LWPKLTPVS}NPJVZ JVTLU[HKVZ tZ[H KLIL
ser alta en esta región.
*\HKYV.0KLU[PKHKLU[YLSPWVJHSPUHZ\ZHUKVHNH[VJVTV
secuencia base
(SPULHTPLU[V
0KLU[PKHK 5‚TKL
5‚TKL
HTPUVmJPKVZHTPUVmJPKVZ
idénticos
ZPTPSHYLZ
.H[VIV]PUV
.H[VJHIHSSV
-LSK,X\J
130
-LSK,X\J
.H[VNHSSPUH
-LSK7\YW\YPUH
16
-LSK7<9
-LSK7\YW\YPUH
36
-LSK7<9
.H[VOmTZ[LY
-LSK7OVZR+H
36
-LSK7OVZR+H
-LSK)VZK
-LSK)VZK
30
60
60
6[YV MHJ[VY PTWVY[HU[L X\L W\LKL YLWLYJ\[PY
en la alta sensibilización a estos animales es
SHYLHJ[P]PKHKJY\aHKHHSHMHTPSPHKLWYV[LxUHZ
SPWVJHSPUHZ,U[YLSHZLZWLJPLZKLWLYYVNH[V`
caballo13 LZ[HZP[\HJP}UMH]VYLJLSHJVZLUZPIPSPaHJP}ULU[YLWHJPLU[LZX\L[PLULUWVJHL_WVZPJP}U
HSVZHUPTHSLZ,UU\LZ[YHWVISHJP}ULZ[VWVKYxH
L_WSPJHY HS TLUVZ LU WHY[L SH HS[H JVZLUZPIPSPaHJP}U H JHIHSSV NH[V ` WLYYV *VU IHZL LU
SVZ HUmSPZPZ IPVPUMVYTm[PJVZ Z\NLYPTVZ X\L SH
JVZLUZPIPSPaHJP}UWVKYxHZLYWVYSHHS[HPKLU[PKHK
LU[YL*HUM-LSK`,X\J+LHJ\LYKVJVU
LSWH[Y}UKLZLUZPIPSPaHJP}U[VKVZSVZWHJPLU[LZ
ZLUZPIPSPaHKVZHJHIHSSVSVLZ[\]PLYVU[HTIPtUH
WLYYVWVYSVX\L,X\JWVKYxHZLYLSWYPUJPWHS
HSLYNLUVKLJHIHSSV7VYSHYLHJ[P]PKHKJY\aHKH
*HUMW\LKLZLY\UPTWVY[HU[LHSLYNLUVWHYH
WLYYVWLYVUVLS‚UPJVWVYX\LSHTH`VYxHKLSVZ
WHJPLU[LZZLUZPIPSPaHKVZHWLYYVUVSVLZ[\]PLYVU
HNH[VVJHIHSSV-LSKWVKYxHZLY\UHSLYNLUV
importante para gato en nuestra población si se
[VTHLUJ\LU[HSHHS[HPKLU[PKHKX\L[PLULJVU
*HUM`SHHS[HMYLJ\LUJPHKLJVZLUZPIPSPaHJP}UH
WLYYVLU[YLSVZWHJPLU[LZZLUZPIPSPaHKVZHNH[V"ZPU
LTIHYNVLZ[HZWYLKPJJPVULZKLILUJVUÄYTHYZL
7
2016 enero-marzo;63(1)
Revista Alergia México
ZLS
K
-L
SPZ
JH
[\
PU
V[L
Y
NW
KPU
IPU
VS
U
L[P
,X\\ZJHIHSS\Z,X\J
ZY
SS\
NH
.
0.2
S\Z
10
W\Y
0
W\Y
M
HU
0
NHS
Z*
PHYP
10
.
TPS
ZMH
PU
UP
*H
Z-LSK
-LSPZJH[\
60
P
H
PSP
U
*H
YPZ
7O
VK
T
*HUM
VW
\Z
Z\
UN
VY
[H
)VZ
\Z
7
OV
Z
2+
K
)VZ
\Y\Z
YPZ*
HUM
PZMHT
PSPH
*HU
U
*H
TPSPHYPZ
M
H
ZM
*HUPZMH
100
-PN\YHÍYIVSÄSVNLUt[PJV ,SmYIVS}W[PTVJVUSHZ\THKLSHSVUNP[\KKLSHYHTHLZPN\HSH WVY Tt[VKVZ in vivo o in vitro 5\LZ[YVZ YLZ\S[HKVZZVUZPTPSHYLZHSVZLUJVU[YHKVZWVYV[YVZ
H\[VYLZSVX\LZ\NPLYL\UHHS[HWYVIHIPSPKHKKL
YLHJ[P]PKHKJY\aHKHKLTVZ[YHKHWVYHUmSPZPZKL
PUOPIPJP}U ` JVTWL[PJP}U \[PSPaHUKV SH [tJUPJH
KL,30:(LU[YL-LSK*HUM`,X\J+L
8
J\HSX\PLYTHULYHSHMYLJ\LUJPHKLZLUZPIPSPaHJP}UHNH[VWLYYV`JHIHSSVLZKPMLYLU[LLU[YLSHZ
WVISHJPVULZWVYSVX\LSVZMHJ[VYLZKLSHTIPLU[L
`SHZJHYHJ[LYxZ[PJHZNLUt[PJHZKLJHKHWVISHJP}U
PUÅ\`LUKLTHULYHPTWVY[HU[LLUSHWYL]HSLUJPH
KLSHZLUZPIPSPaHJP}UHKPMLYLU[LZM\LU[LZ
Re vista
Sánchez A y col. Análisis in silico de lipocalinas en alergia a mascotas
*\HKYV *HYHJ[LYxZ[PJHZNLULYHSLZKLSHWVISHJP}UU$
+H[VZNLULYHSLZ
5‚T
:L_V
4\QLY
/VTIYL
-LUV[PWVZ
(ZTH
9PUP[PZ
*VUQ\U[P]P[PZ
Eccema
,KHKH|VZ
4LKPHSxTP[LZ!H
*\HKYV *HYHJ[LYxZ[PJHZKLSHWVISHJP}UWVYZLUZPIPSPaHJP}U
U$
+H[VZNLULYHSLZ
([VWPH
7LYYV
.H[V
7mQHYVZ
*HUHYPVZWLYPJVZWHSVTHZ`NHSSPUHZ
6[YVZHUPTHSLZ
*HIHSSVJVULQVOmTZ[LYJLYKV
5‚T
U$U$ *VTWHYHKV JVU V[YHZ LZWLJPLZ ZL ZHIL WVJV
HJLYJHKLSHMHTPSPHKLSPWVJHSPUHZ`Z\ZWYVWPLKHKLZHSLYNtUPJHZLUSHNHSSPUH]HJH`OmTZ[LY16
6IZLY]HTVZ \UH IHQH JVZLUZPIPSPaHJP}U H
OmTZ[LYV]HJHJVUNH[VVWLYYV,Z[HIHQHJVZLUZPIPSPaHJP}UJVUJ\LYKHJVUSHIHQHPKLU[PKHK
LU[YLSHZSPWVJHSPUHZKLLZ[HZLZWLJPLZ`Z\TH`VY
KPZ[HUJPH ÄSVNLUt[PJH ,_PZ[LU WVJVZ LZ[\KPVZ
HJLYJHKLSHZLUZPIPSPaHJP}UHSVZWmQHYVZ
6IZLY]HTVZX\LSHMYLJ\LUJPHKLZLUZPIPSPaHJP}U
HWmQHYVZJVTVNY\WVM\LTH`VYKLWLYV
SHZLUZPIPSPaHJP}UHNHSSPUHWHSVTHWLYPX\P[VV
JHUHYPVZLWHYHKHTLU[LM\LTLUVYKL Esta
IHQHMYLJ\LUJPHKLZLUZPIPSPaHJP}UJVTWHYHKH
JVUSHKLNH[VVWLYYVW\LKLKLILYZLHX\LWVJHZWLYZVUHZLUU\LZ[YHWVISHJP}U[PLULUWmQHYVZ
JVTVTHZJV[HWLYV[HTIPtUW\LKLKLILYZLHSH
WVJHPKLU[PKHKJVUSHZSPWVJHSPUHZKLV[YHZLZWLJPLZJVTVNH[VVWLYYV3HL_WVZPJP}UKPYLJ[H
WHYLJL[LULYTH`VYPTWVY[HUJPHLUSHZLUZPIPSP-
Mé xic o
aHJP}U H WmQHYVZ X\L SH YLHJ[P]PKHK JY\aHKH H
V[YHZLZWLJPLZ:PULTIHYNVZ}SV[VTHTVZSHZ
SPWVJHSPUHZKLNHSSPUHKLIPKVHX\LOHZ[HU\LZ[YVJVUVJPTPLU[VZVUSHZ‚UPJHZYLWVY[HKHZHS
TVTLU[VWVYSVX\LUVWVKLTVZHZLN\YHYX\L
SHIHQHPKLU[PKHKVIZLY]HKHJVUNH[V`WLYYVZL
L_[PLUKHHV[YHZLZWLJPLZKLWmQHYVZ
5\LZ[YVLZ[\KPV[PLULHSN\UHZMVY[HSLaHZ`SPTP[HJPVULZ+LIPKVHX\LLZ\ULZ[\KPVin silico, las
HZVJPHJPVULZLUJVU[YHKHZLU[YLSVZWH[YVULZKL
JVZLUZPIPSPaHJP}U`SHPKLU[PKHKKLSHZSPWVJHSPUHZ
UVZWLYTP[LL_WSVYHYSVZHSLYNLUVZX\LW\LKLU
[LULY TH`VY WVZPIPSPKHK KL ZLY JSxUPJHTLU[L
YLSL]HU[LZ LU U\LZ[YH WVISHJP}U Z\ LMLJ[V LU
SHYLHJ[P]PKHKJY\aHKHLPKLU[PÄJHYSHZWVZPISLZ
M\LU[LZ WYPUJPWHSLZ KL ZLUZPIPSPaHJP}U :PU LTIHYNVLZ[VZYLZ\S[HKVZHSZLYWYLKPJ[P]VZKLILU
JVUÄYTHYZLJVULZ[\KPVZin vitro WHYHYH[PÄJHYX\L
SVZHSLYNLUVZPKLU[PÄJHKVZZVUSVZJH\ZHU[LZKL
SHYLHJ[P]PKHKJY\aHKH`SHZMYLJ\LUJPHZKLZLUZPIPSPaHJP}ULUJVU[YHKHZ"ZPULTIHYNVtZ[LZLYxHLS
WYPTLYWHZVWHYH\UHI‚ZX\LKHin vitroKPYPNPKH
CONCLUSIÓN
+LHJ\LYKVJVUSVZHUmSPZPZIPVPUMVYTm[PJVZ`
SVZWH[YVULZKLZLUZPIPSPaHJP}ULU\UHWVISHJP}U
KLS[Y}WPJVWYVWVULTVZX\LHSN\UHZSPWVJHSPUHZ
W\LKLU[LULY\UPTWVY[HU[LWHWLSLUSHJVZLUZPIPSPaHJP}UHNH[VWLYYV`JHIHSSVWLYVUVWHYH
OmTZ[LY]HJHVWmQHYVZ-LSK`,X\JWVKYxHU
ZLYHSLYNLUVZTH`VYLZWHYHJHIHSSV`NH[V
Agradecimientos
,S.Y\WVKL(SLYNVSVNxH*SxUPJH`,_WLYPTLU[HS
.(*,KLSH<UP]LYZPKHKKL(U[PVX\PH4LKLSSxU*VSVTIPHÄUHUJP}LZ[LLZ[\KPV
REFERENCIAS
1.
Dennis RJ, Caraballo L, García E, Rojas MX, et al. Prevalence
ŽĨĂƐƚŚŵĂĂŶĚŽƚŚĞƌĂůůĞƌŐŝĐĐŽŶĚŝƟŽŶƐŝŶŽůŽŵďŝĂϮϬϬϵͲ
ϮϬϭϬ͗ĂĐƌŽƐƐͲƐĞĐƟŽŶĂůƐƚƵĚLJ͘DWƵůŵDĞĚϮϬϭϮ͖ϭϮ͗ϭϳ͘
9
Revista Alergia México
2.
Macías Weinmann A, Escamilla Weinmann C, Pazos Salazar
E'͕ sĂůĚĠƐ ƵƌŶĞƐ ͕ 'ŽŶnjĄůĞnj şĂnj ^E͘ ΀^ĞŶƐŝƟǀŝƚLJ ƚŽ
animals’ allergens in people working with animals]. Rev
ůĞƌŐDĞdžϮϬϭϬ͖ϱϳ͗ϭϴϱͲϭϴϵ͘
12. Liccardi G, Salzillo A, Piccolo A, D’Amato G. Skin prick test
to horse should be included in the standard panel for the
ĚŝĂŐŶŽƐŝƐ ŽĨ ƌĞƐƉŝƌĂƚŽƌLJ ĂůůĞƌŐLJ͘ : /ŶǀĞƐƟŐ ůůĞƌŐŽů ůŝŶ
/ŵŵƵŶŽůϮϬϭϬ͖ϮϬ͗ϵϯͲϵϰ͘
ϯ͘
ĚĞ:ŽŶŐ͕ŝŬŬĞƐĐŚĞŝ>͕ƌĂŶĚW>͘^ĞŶƐŝƟnjĂƟŽŶƉĂƩĞƌŶƐ
to food and inhalant allergens in childhood: a comparison
of non-sensitized, monosensitized, and polysensitized
children. Pediatr Allergy Immunol 2011;22:166-171.
ϭϯ͘ ŽƌƌĞƐ DW͕ ďŝƐĂǁĂ D͕ ŝŐĞŶŵĂŶŶ W͘ hƐĞ ŽĨ ĂůůĞƌŐĞŶ
components begins a new era in pediatric allergology.
Pediatr Allergy Immunol 2011;22:454-461.
ϰ͘
ƵƌďĂĐŚ'͕,ĞŝŶnjĞƌůŝŶŐ>͕ĚĞŶŚĂƌƚĞƌ'͕ĂĐŚĞƌƚ͕ĞƚĂů͘
GA(2)LEN skin test study II: clinical relevance of inhalant
ĂůůĞƌŐĞŶ ƐĞŶƐŝƟnjĂƟŽŶƐ ŝŶ ƵƌŽƉĞ͘ ůůĞƌŐLJ ϮϬϬϵ͖ϲϰ͗ϭϱϬϳͲ
1515.
ϭϰ͘ ,ŝůŐĞƌ͕<ƵĞŚŶ͕,ĞŶƚŐĞƐ&͘ŶŝŵĂůůŝƉŽĐĂůŝŶĂůůĞƌŐĞŶƐ͘
Curr Allergy Asthma Rep 2012;12:438-447.
ϭϱ͘ EŝůƐƐŽŶK͕ŝŶŶŵLJƌ:͕ŽůƚŽǁƐŬĂ͕^ĂĂƌŶĞd͕ĞƚĂů͘ŚĂƌĂĐƚĞƌŝnjĂƟŽŶŽĨƚŚĞĚŽŐůŝƉŽĐĂůŝŶĂůůĞƌŐĞŶĂŶĨϲ͗ƚŚĞƌŽůĞŝŶ
ĐƌŽƐƐͲƌĞĂĐƟǀŝƚLJǁŝƚŚĐĂƚĂŶĚŚŽƌƐĞ͘ůůĞƌŐLJϮϬϭϮ͖ϲϳ͗ϳϱϭͲϳϱϳ͘
5.
Sánchez J, Diez S, Cardona R. Frecuencia de sensibilización a
ĂŶŝŵĂůĞƐĞŶƵŶĄƌĞĂƚƌŽƉŝĐĂů͘ZĞǀůĞƌŐDĞdžϮϬϭϰ͖ϲϭ͗ϴϭͲϴϵ͘
ϲ͘
ƌŽnjĞŬ:>͕ŽƵƐƋƵĞƚ:͕ĂĞŶĂͲĂŐŶĂŶŝ͕ŽŶŝŶŝ^͕ĞƚĂů͘
ůůĞƌŐŝĐZŚŝŶŝƟƐĂŶĚŝƚƐ/ŵƉĂĐƚŽŶƐƚŚŵĂ;Z/ͿŐƵŝĚĞůŝŶĞƐ͗
2010 revision. J Allergy Clin Immunol 2010;126:466-476.
ϭϲ͘ dŽƌƌĞƐ :͕ ĚĞ >ĂƐ ,ĞƌĂƐ D͕ DĂƌŽƚŽ ^͕ sŝǀĂŶĐŽ &͕ Ğƚ Ăů͘
Molecular and immunological characterization of the
ĮƌƐƚ ĂůůĞƌŐĞŶŝĐ ůŝƉŽĐĂůŝŶ ŝŶ ŚĂŵƐƚĞƌ͗ ƚŚĞ ŵĂũŽƌ ĂůůĞƌŐĞŶ
from Siberian hamster (Phodopus sungorusͿ͘:ŝŽůŚĞŵ
ϮϬϭϰ͖Ϯϴϵ͗ϮϯϯϴϮͲϮϯϯϴϴ͘
ϳ͘
^ĂŶƚŽƐ D^͕ ůǀĞƐ DZ͕ &ƌĞŝƚĂƐ ͕ ^ŽƵƐĂ >͕ Ğƚ Ăů͘ KĐƵůĂƌ
ĂůůĞƌŐLJ >ĂƚŝŶ ŵĞƌŝĐĂŶ ĐŽŶƐĞŶƐƵƐ͘ ƌƋ ƌĂƐ KĨƚĂůŵŽů
2011;74:452-456.
17. Chapman JA, Williams S. Aeroallergens of the southeast
DŝƐƐŽƵƌŝ ĂƌĞĂ͗ Ă ƌĞƉŽƌƚ ŽĨ ƐŬŝŶ ƚĞƐƚ ĨƌĞƋƵĞŶĐŝĞƐ ĂŶĚ Ăŝƌ
ƐĂŵƉůŝŶŐĚĂƚĂ͘ŶŶůůĞƌŐLJϭϵϴϰ͖ϱϮ͗ϰϭϭͲϰϭϴ͘
ϴ͘
,ĂŶŝĮŶ:D͘ŝĂŐŶŽƐƟĐĐƌŝƚĞƌŝĂĨŽƌĂƚŽƉŝĐĚĞƌŵĂƟƟƐ͗ĐŽŶƐŝĚĞƌƚŚĞĐŽŶƚĞdžƚ͘ƌĐŚĞƌŵĂƚŽůϭϵϵϵ͖ϭϯϱ͗ϭϱϱϭ͘
ϵ͘
,ĞŝŶnjĞƌůŝŶŐ>D͕ƵƌďĂĐŚ':͕ĚĞŶŚĂƌƚĞƌ'͕ĂĐŚĞƌƚ͕Ğƚ
Ăů͘';ϮͿ>EƐŬŝŶƚĞƐƚƐƚƵĚLJ/͗';ϮͿ>EŚĂƌŵŽŶŝnjĂƟŽŶŽĨ
ƐŬŝŶƉƌŝĐŬƚĞƐƟŶŐ͗ŶŽǀĞůƐĞŶƐŝƟnjĂƟŽŶƉĂƩĞƌŶƐĨŽƌŝŶŚĂůĂŶƚ
ĂůůĞƌŐĞŶƐŝŶƵƌŽƉĞ͘ůůĞƌŐLJϮϬϬϵ͖ϲϰ͗ϭϰϵϴͲϭϱϬϲ͘
ϭϴ͘ >ŝŶŶĂ K͕ EŝŝŶŝŵćŬŝ ͕ DćŬŝŶĞŶͲ<ŝůũƵŶĞŶ ^͘ /ŵŵƵŶŽůŽŐŝĐ
ĐƌŽƐƐͲƌĞĂĐƟǀŝƚLJ ďĞƚǁĞĞŶ ŚĞŶ͛Ɛ ĨĞĂƚŚĞƌ ĂŶĚ ŚŽƵƐĞͲĚƵƐƚͲ
ŵŝƚĞĂůůĞƌŐĞŶĞdžƚƌĂĐƚƐ͘ůůĞƌŐLJϭϵϵϰ͖ϰϵ͗ϳϵϱͲϳϵϲ͘
10. De Knop KJ, Verweij MM, Grimmelikhuijsen M, Philipse E,
ĞƚĂů͘ŐĞͲƌĞůĂƚĞĚƐĞŶƐŝƟnjĂƟŽŶƉƌŽĮůĞƐĨŽƌŚĂnjĞůŶƵƚ;Corylus
avellana) in a birch-endemic region. Pediatr Allergy ImmuŶŽůϮϬϭϭ͖ϮϮ;ϭWƚϮͿ͗ĞϭϯϵͲϰϵ͘
ϭϭ͘ DĂƚƌŝĐĂƌĚŝWD͕ŽĐŬĞůďƌŝŶŬ͕ĞLJĞƌ<͕<Ğŝůd͕ĞƚĂů͘WƌŝŵĂƌLJ
versusƐĞĐŽŶĚĂƌLJŝŵŵƵŶŽŐůŽďƵůŝŶƐĞŶƐŝƟnjĂƟŽŶƚŽƐŽLJ
ĂŶĚǁŚĞĂƚŝŶƚŚĞDƵůƟͲĞŶƚƌĞůůĞƌŐLJ^ƚƵĚLJĐŽŚŽƌƚ͘ůŝŶ
džƉůůĞƌŐLJϮϬϬϴ͖ϯϴ͗ϰϵϯͲϱϬϬ͘
10
2016 enero-marzo;63(1)
ϭϵ͘ <ĞŵƉd:͕^ŝĞďĞƌƐZt͕&ŝƐŚǁŝĐŬ͕K͛'ƌĂĚLJ'͕ĞƚĂů͘,ŽƵƐĞ
ĚƵƐƚŵŝƚĞĂůůĞƌŐĞŶŝŶƉŝůůŽǁƐ͘D:ϭϵϵϲ͖ϯϭϯ͗ϵϭϲ͘
ϮϬ͘ ŽůůŽīD:͕DĞƌƌĞƩd'͕DĞƌƌĞƩ:͕DĐ^ŚĂƌƌLJ͕ŽLJĚ'͘&ĞĂƚŚĞƌ
ŵŝƚĞƐĂƌĞƉŽƚĞŶƟĂůůLJĂŶŝŵƉŽƌƚĂŶƚƐŽƵƌĐĞŽĨĂůůĞƌŐĞŶƐĨŽƌƉŝŐĞŽŶ
ĂŶĚďƵĚŐĞƌŝŐĂƌŬĞĞƉĞƌƐ͘ůŝŶdžƉůůĞƌŐLJϭϵϵϳ͖Ϯϳ͗ϲϬͲϲϳ͘
Ϯϭ͘ <ŝůƉŝƂ<͕DćŬŝŶĞŶͲ<ŝůũƵŶĞŶ^͕,ĂĂŚƚĞůĂd͕,ĂŶŶƵŬƐĞůĂD͘
ůůĞƌŐLJƚŽĨĞĂƚŚĞƌƐ͘ůůĞƌŐLJϭϵϵϴ͖ϱϯ͗ϭϱϵͲϭϲϰ͘
22. Sanchez J, Diez S, Cardona R. Sensibilización a aeroalergenos en pacientes alérgicos de Medellín, Colombia. Rev
ůĞƌŐDĠdžϮϬϭϮ͖ϱϵ͗ϭϯϵͲϭϰϳ͘